The double resistance enrichment medium (3) consists of 1 ml of 100 mg/ml double resistance solution (penicillin and streptomycin) per 100 ml YEPD medium.
ussuriensis Maxim fruit were cut using a sterile knife and placed in the double resistance enrichment medium. The peels were cultured at 28 [degrees]C with shaking at 100 r/min for 24 h.
The supernatant (extract) was collected and used for the preparation of enrichment medium
. The enrichment medium
used for bacterial isolation was composed of sludge extract and nutrient broth in the ratio of 1:2.
When using a detection system, a proper selective enrichment medium
is needed to detect multiple pathogens in a food sample.
Following 4 days of incubation, the enrichment medium
became noticeably more turbid as a result of cell growth in all tubes except those inoculated with sediments from sites 21 and 41 (no detectable TCE degradation at these sites).
For all three packages (steak, butterfly cut, and sausage pieces), a combined weight of 25 g frozen meat shavings were macerated, incubated in enrichment medium
, and immunomagnetically separated (IMS), according to the manufacturers' instructions (Dynal, Inc., Lake Success, NY).
Addition of serum to the enrichment medium
used to culture V.
It then also serves as a selective enrichment medium
for the isolation of E.
Fraser Broth is traditionally used as a secondary enrichment medium
for the isolation of all Listeria species.