Parasito N % IC95% Endolimax nana 28 6,5 4,4 - 9,4 Entamoeba
coli 27 6,3 4,2 - 9,1 Giardia intestinalis 15 3,5 2,0 - 5,8 Ascaris lumbricoides 6 1,4 0,6 - 3,2 Strongyloides stercoralis 3 0,7 0,2 - 2,2 Enterobius vermicularis 3 0,7 0,2 - 2,2 Ancilostomideo 1 0,2 0,0 - 1,5 Entamoeba
hystolitica 1 0,2 0,0 - 1,5 Taenia sp 1 0,2 0,0 - 1,5 Total de exames positivos 69 16,0 12,7 - 19,9 Tabela 2.
Amoebiasis is a ubiquitous disease, endemic to Asia, Africa and South America, is an infection by Entamoeba histolytica, affecting young to middle-aged adults.
and metronidazole against Entamoeba histolytica HM1 cells Total no.
histolytica genes and identification of mRNAs encoding Fehydrogenase from cultured entamoebas and giardias.
Total RNA was prepared by lysing cultured entamoebas and giardias in a guanidinium isothiocyanate solution and by centrifuging the lysate through a cesium chloride gradient (Choczynski and Sacchi, 1987).
vaginalis Fe-hydrogenase was greater than that of the recombinant entamoebic GST-entamoebic Fe-hydrogenase and was inhibited by a lysate of non-transfected entamoebas (Table 1).
Cultured entamoebas and giardias express mRNAs encoding short Fe-hydrogenases.
The entamoebic Fe-hydrogenase 2 was much more similar (>38% amino acid identities) to predicted long Fe-hydrogenases of Bacteroides fragilis and Treponema denticola than to short Fe-hydrogenases of entamoebas, giardias, trichomonads, and other anaerobic bacteria (<28% amino acid identities; Fig.
This lateral gene transfer would not have occurred recently, because the Fe-hydrogenases of entamoebas and giardias showed only a 40% amino acid identity with each other, and each fe-hydrogenase gene has the codon usage of its host.