enzyme inhibition


Also found in: Medical.

Enzyme inhibition

The prevention of an enzymic process as a result of the interaction of some substance with an enzyme so as to decrease the rate of the enzymic reaction. The substance causing such an effect is termed an inhibitor. Enzyme inhibitors are important as chemotherapeutic agents, as regulators in normal control of enzymic processes in living organisms, and as useful agents in the study of biochemistry. See Antibiotic, Chemotherapy, Enzyme

Inhibitors have been classified as competitive, noncompetitive, and uncompetitive. The effect of a competitive inhibitor is to bind only free enzyme. This can be reversed by sufficiently increased substrate concentrations, so that essentially all of the enzyme is bound into an enzyme-substrate complex. Since both noncompetitive and uncompetitive inhibitors interact with the enzyme-substrate complex, their effects are not nullified by increased concentrations of substrate. An uncompetitive inhibitor exerts less effect (as percent of control) at low than at high substrate concentrations, since less of the enzyme is in the form of the enzyme-substrate complex, with which it interacts. A noncompetitive inhibitor, which reacts with both free enzyme and the enzyme-substrate complex, exerts comparable effects at all substrate concentrations.

enzyme inhibition

[′en‚zīm ‚in·ə·′bish·ən]
(biochemistry)
Prevention of an enzymic process as a result of the interaction of some substance with the enzyme so as to decrease the rate of reaction.
References in periodicals archive ?
Khan H, Ali Khan M, Hussain I (2007) Enzyme inhibition activities of the extracts from rhizomes of Gloriosa superba Linn (Colchicaceae).
Angiotensin converting enzyme inhibition lowers body weight and improves glucose tolerance in C57BL/6J mice maintained on a high Fat diet.
The latest findings by the researchers at the UT Southwestern Medical Center demonstrated that telomerase repairs chromosomes in one of two ways - depending on whether a cell is dividing normally or if the cell is under stress from enzyme inhibition - and could lead to new or improved cancer-fighting therapies that promote inhibition of this enzyme.
To further characterize the inhibition of CYP enzymes activity by tanshinones, enzyme inhibition kinetic experiments were carried out.
Enzyme inhibition in drug discovery and development; the good and the bad.
265) developed a mechanistik computational model to estimate the synthesis and secretion of 14 adrenal steroids in H295R cells and biochemical responses to enzyme inhibition by the model EAC metryrapone (MET), and they compared model estimates with time-course measurements in control and MET-exposed H295R cells.
From these initial studies, the scientists found that soy and blueberry base in yogurt has the best enzyme inhibition potential.
The earlier Heart Outcomes Prevention Evaluation (HOPE), European Trial on Reduction of Cardiac Events With Perindopril in Stable CAD (EUROPA), and Prevention of Events With Angiotensin-Converting Enzyme Inhibition (PEACE) trims included collectively more than 9,200 patients who had undergone CABG, and those patients experienced a significant reduction in ischemic events.
The earlier Heart Outcomes Prevention Evaluation (HOPE), European Trial on Reduction of Cardiac Events With Perindopril in Stable CAD (EUROPA), and Prevention of Events With Angiotensin-Converting Enzyme Inhibition (PEACE) trials included collectively more than 9,200 patients who had undergone CABG, and those patients experienced a significant reduction in ischemic events.
The Birmingham University study into the effects of selective enzyme inhibition may also benefit people with metabolic disorders, which can cause sufferers to become obese.
Another study, the Angiotensin-converting Enzyme Inhibition Post-Revascularization Study, evaluated treatment with ramipril following PTCA or coronary artery bypass grafting in 159 normotensive patients with reduced ejection fraction (0.
The Galileo LIMS system is designed to conduct permeability, enzyme inhibition (IC50 and Ki) assays, metabolic stability and profiling, and protein binding experiments in a GLP and 21 CFR Part 11 compliant manner.