The dye detector retrieved at the pipe seven days later tested positive for fluorescein, as well as the eosine
used in one of the previous tracing attempts.
The sample was inoculated with a 0.001 ml calibrated loop on to a BA and an eosine
methylene blue agar (EMB) as selective medium for Gram-negatives .
Nutrient agar, Maconkey agar (MCA), Eosine
Methylene blue agar (EMB), Blood agar, Brain and heart infusion (BHI) agar, Clostridial agar, Egg yolk agar, Manitol salt agar (MSA) and Xylose lysine decarboxylase (XLD) agar were used as primary culture media and selective culture medias for isolation of various bacterial organisms according to the methods described by Quinn et al.
Formalin fixed tissues were embedded in paraffin and core region of tissues (5um thickness) were mounted on the glass slides and stained with Hematoxline and Eosine
. Histopathological examination of tumors were performed by veterinary pathologist according to the criteria of the WHO classification of canine and feline tumors.
Sections 5 [micro]m thick were stained with Hematoxilin and Eosine
Mayer's staining method (Bacha & Wood, 1990).
Tissue sections were stained with haematoxyline and eosine
and PAS stains.
Smears were air dried and wet fixed and stained with hematoxyline and eosine
, Giemsa and Z-N stain for AFB.
The blood cultures which gave positive growth were gram stained and planted in 5% sheep blood agar, eosine
methylene blue (EMB) agar, chocolate agar and Sabouraud dextrose-agar (SDA) media.
Table 4--Stain Resistance of Cured Coatings (a) Stain Formulation A Formulation B Formulation C Resistance Emulsion Reference Reference Test Water-based Solvent-based UV-PUD RC Oligomer #1 Black shoe 5 5 5 polish #2 Tar 5 5 5 #3 Black marker 5 5 5 (Artline[R] 70 N) #4 Blue 5 5 5 colorant ( BB750 H20) #5 Sudan red 5 5 5 colorant ( SR380-WS) #6 Yellow 5 5 5 colorant ( SG146-WS) #7 Eosine
5 5 5 #8 4.5 4 5 Iso-betadine Average stain 4.9 4.9 5 resistance (a) Ranking from 1 = severe trace visible to 5 = no trace Table 5 presents the water resistance of the cured coatings on polycarbonate and acrylonitrile butadiene styrene (ABS) using three test protocols, namely (24 hr; RT), (2 hr; 80[degrees]C) and (72 hr; 90[degrees]C; 95% RH).
Coliform bacteria were carried out on VRBL agar medium and incubated for 24 hours at 37 [degrees]C for total coliforms and 44 [degrees]C for fecal coliforms according to the standard (ISO 4832); E.coli was streaked onto eosine
methylene blue (EMB) agar and then incubated overnight at 37[degrees]C.
Tissues were prepared following the technique of hematoxiline and eosine
(Castro & Wourms 1993); this technique was carried out in the Invertebrates Laboratory at CICIMAR.
Serial sections (2-3 [micro]m thick) were stained with toluidine blue (Spurlock et al., 1966), methylene blue/ basic fuchsin (Bennett et al., 1976), or hematoxiline/ eosine