Caption: FIGURE 2: Effect of BA, with and without IBA or NAA, on the shoot regeneration rate in explants from epicotyls
6%), thickening of hypocotyls, strong reduction of epicotyls
and roots with few or no lateral root formation (Fig.
(Figure 4A) consists of uniseriate epidermis with unicellular non-glandular trichomes, collenchymatous and parenchymatous cortex, and central cylinder composed of two collateral vascular bundles and phloematic strands that surround the parenchymatous pith.
Thus, the minimum optimal number of plants to be measured to represent the average hypocotyl length was 45, 53, 52 and 45 for BRS Valiosa RR, Agua-Marinha RR, UFVS 2010 and NK 7059 RR, respectively, whereas the average length of the epicotyls
was 49, 43, 51 and 49 for BRS Valiosa RR, Agua-Marinha RR, UFVS 2010 and NK 7059 RR, respectively (Figure 1).
In conclusion, in terms of number of callus and hormone reactivity, epicotyls
and hypocotyls explants and zygotic embryos were the most suitable explants material.
Data were collected on fresh weight (FW, g) of leaf blade (LFW), and on FW and length (L, cm) of the epicotyl
(EL and EFW), hypocotyl (HL and HFW), first leaf petiole (FL and FFW), trifoliate leaf petiole (TL and TFW) and root (RL and RFW).
Growth and cell wall changes in azuki bean epicotyls
The longest epicotyls
were observed in response to etiolating of plants to reach the soil surface.
Parrott (2000) A protocol for repetitive somatic embryogenesis from mature peanut epicotyls
Most citrus in vitro organogenesis protocols are based on the culture of young tissue, mainly epicotyls
and intermodal segments (GARCIA-LUIS et al.
The main root length was measured, as well as hypocotyls, epicotyls
, and shoot; the total biomass of the seedling and the cotyledonal area of the eophylls.
Dopico B, Nicolas G, Labrador E (1989) Partial purification of cell wall [beta]-galactosidases from Cicer arietinum epicotyls