equilibrium dialysis


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equilibrium dialysis

[‚ē·kwə′lib·rē·əm dī′al·ə·səs]
(analytical chemistry)
A technique used to determine the degree of ion bonding by protein; the protein solution, placed in a bag impermeable to protein but permeable to small ions, is immersed in a solution containing the diffusible ion whose binding is being studied; after equilibration of the ion across the membrane, the concentration of ion in the protein-free solution is determined; the concentration of ion in the protein solution is determined by subtraction; if binding has occurred, the concentration of ion in the protein solution must be greater.
References in periodicals archive ?
The use of a sensitive equilibrium dialysis method for the measurement of free testosterone levels in healthy, cycling women and in human immunodeficiency virus-infected women.
This was accomplished by progressively diluting serum retentate with serum dialysate (obtained using the equilibrium dialysis method described above).
A theoretically sound and practicable equilibrium dialysis method for measuring percentage of free testosterone.
Normal free thyroxine in critical nonthyroidal illnesses measured by ultrafiltration of undiluted serum and equilibrium dialysis.
Ultrafltration devices tested for use in a free thyroxine assay validated by comparison with equilibrium dialysis.
4] was assessed by RIA of the dialysate after equilibrium dialysis and was found to be normal (22 ng/L; reference interval, 8-27 ng/L; Nichols Institute).
A simplified method for the calculation of unbound or free testosterone by equilibrium dialysis of undiluted plasma.
Equilibrium dialysis was performed using an assay from Nichols Institute Diagnostics.
Such experiments, in which (unlike the case with equilibrium dialysis or ultrafiltration) the serum binding proteins, their [T.
Discrepancies between serum free triiodothyronine and free thyroxin as measured by equilibrium dialysis and analog radioimmunoassay in nonthyroidal illnesses.
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