equilibrium dialysis


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equilibrium dialysis

[‚ē·kwə′lib·rē·əm dī′al·ə·səs]
(analytical chemistry)
A technique used to determine the degree of ion bonding by protein; the protein solution, placed in a bag impermeable to protein but permeable to small ions, is immersed in a solution containing the diffusible ion whose binding is being studied; after equilibration of the ion across the membrane, the concentration of ion in the protein-free solution is determined; the concentration of ion in the protein solution is determined by subtraction; if binding has occurred, the concentration of ion in the protein solution must be greater.
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Free thyroid hormones in serum by direct equilibrium dialysis and online sold-phase extraction-liquid chromatography/tandem mass spectrometry.
Effects of ILE on Free Drug Concentration in Human Whole Blood Using Equilibrium Dialysis. Bupivacaine showed a statistically significant reduction in percent fall of drug concentration compared to the other LA agents at all ILE concentrations (Figure 2).
Free thyroxine measured by equilibrium dialysis and nine immunoassays in sera with various serum thyroxine-binding capacities.
New ultrafiltration method for free thyroxin compared with equilibrium dialysis in patients with thyroid dysfunction and nonthyroidal illness.
The use of a sensitive equilibrium dialysis method for the measurement of free testosterone levels in healthy, cycling women and in human immunodeficiency virus-infected women.
In the first experiment, preparative equilibrium dialysis was applied to the normal adult male serum as described above (200 [micro]L retentate vs 2400 [micro]L dialysate).
Finally, although ultrafiltration, equilibrium dialysis, and direct analog assays have the same aims, in regard to sampling and dilution these different assays represent opposite ends of a spectrum of valid methods.
When dialysis was applied, the pH of serum dialysate and retentate was controlled to mean (SD) 7.4 (0.1) during equilibrium dialysis at 37 [degrees]C by the HEPES acid in dialysate buffer (27).
Direct equilibrium dialysis and direct ultrafiltration free thyroxine ([T.sub.4]) methods use semipermeable membranes to separate free [T.sub.4] from [T.sub.4]-binding serum proteins.
The measurement procedure to be used for that purpose must include physical separation of free and protein-bound [T.sub.4], either by equilibrium dialysis (ED) or ultrafiltration (UF).
Measurement of the serum [T.sub.4] after equilibrium dialysis is the most accurate method to remove potential [T.sub.4] -binding protein interferences (18), although a report by Hoshikawa et al.
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