Free thyroid hormones in serum by direct equilibrium dialysis
and online sold-phase extraction-liquid chromatography/tandem mass spectrometry.
Effects of ILE on Free Drug Concentration in Human Whole Blood Using Equilibrium Dialysis
. Bupivacaine showed a statistically significant reduction in percent fall of drug concentration compared to the other LA agents at all ILE concentrations (Figure 2).
Free thyroxine concentrations in serum measured by equilibrium dialysis
in chronic renal failure.
Free thyroxine measured by equilibrium dialysis
and nine immunoassays in sera with various serum thyroxine-binding capacities.
New ultrafiltration method for free thyroxin compared with equilibrium dialysis
in patients with thyroid dysfunction and nonthyroidal illness.
The use of a sensitive equilibrium dialysis
method for the measurement of free testosterone levels in healthy, cycling women and in human immunodeficiency virus-infected women.
In the first experiment, preparative equilibrium dialysis
was applied to the normal adult male serum as described above (200 [micro]L retentate vs 2400 [micro]L dialysate).
Finally, although ultrafiltration, equilibrium dialysis
, and direct analog assays have the same aims, in regard to sampling and dilution these different assays represent opposite ends of a spectrum of valid methods.
When dialysis was applied, the pH of serum dialysate and retentate was controlled to mean (SD) 7.4 (0.1) during equilibrium dialysis
at 37 [degrees]C by the HEPES acid in dialysate buffer (27).
Direct equilibrium dialysis
and direct ultrafiltration free thyroxine ([T.sub.4]) methods use semipermeable membranes to separate free [T.sub.4] from [T.sub.4]-binding serum proteins.
The measurement procedure to be used for that purpose must include physical separation of free and protein-bound [T.sub.4], either by equilibrium dialysis
(ED) or ultrafiltration (UF).
Measurement of the serum [T.sub.4] after equilibrium dialysis
is the most accurate method to remove potential [T.sub.4] -binding protein interferences (18), although a report by Hoshikawa et al.