In this study we used a direct injection of Ethidium
bromide (EB) into the intracerebral ventricle (ICV) as a simple model for induction of neural cells degeneration and MS disease.
PCR was performed and its products were electrophoresed in an ethidium
bromide stained 1% agarose gel with I>>-Hind III digest as a marker 1.
The Office of Campus Sustainability also highly recommends lab usage of Gel Red as part of its initiatives--a safer alternative in DNA research to toxic ethidium
bromide--as well as the usage of energy efficient freezers typically used in biochemistry, biology and life sciences laboratories.
DNA staining in gels has historically been carried out using silver staining and fluore-scent dyes like ethidium
bromide and SYBR Green I (SGI)5.
The amplification products were electrophoresed through a 2% agarose gel and visualized with UV transilluminator after ethidium
This helps to guarantee high and consistent recovery rates in excess of 85 percent, while simultaneously removing primer-dimers, primers, nucleotides, proteins, salt, agarose, ethidium
bromide, and other impurities.
The slides were then stained with ethidium
bromide and observed under fluorescent microscope.
bromide (10 mg) was mixed with 10 mL of sterile distilled water.
bromide was purchased from Merck KGaA (Germany).
Each material was tested on human peripheral blood lymphocytes using the micronucleus assay and ethidium
bromide/acridine orange viability staining.
In this connection, wild bacterial strain Streptococcus equisimilis was mutagenized using ethidium
bromide and UV radiation.