The fragments were separated by agarose gel electrophoresis and visualized with ethidium bromide
. The -1584 G homozygote was diagnosed by a 131-bp fragment (and a small 20-bp fragment not distinguishable from the primers); the -1584 C homozygote PCR product remained uncut (data not shown).
The amplification product were separated in 1.2% agarose gel and stained with Ethidium bromide
. Agarose gel showed good resolution to score the band.
3 shows the morphology of U937, HL60 and K562 cells treated with ME and EAF and stained with acridine orange and ethidium bromide
. The fluorescent images showed that the untreated cells possessed intact nuclei and that the treatment caused chromatin condensation, membrane blebbing and formation of apoptotic bodies.
Stain with 25 [micro]l of 10 mg/ml ethidium bromide
, shaking for three minutes.
DNA was collected by centrifugation (4 [degrees]C, 29 000 X g, 20 min) and analyzed by electrophoresis in 1.2% agarose gel containing 0.1% ethidium bromide
in TAE buffer (60 v, 2 h).
At the conclusion of the run, gels are bathed in ethidium bromide
and photographed by a charge-coupled device digital camera system (3, 4).
We determined the concentration and purity of samples using spectrophotometer scan in the UV region and ethidium bromide
(EtBr) visualization of intact 18S and 28S RNA bands following agarose gel electrophoresis.
After the amplification steps were completed, the reaction mixtures were exposed to a final elongation step at 72[degrees]C for 7 min, and PCR products were visualized with ethidium bromide
(GIBCO BRL Life Technologies, Inc., Gaithersburg, MD, USA) on 1.5% agarose gels after electrophoresis.
RT-PCR products were resolved by 2% gel electrophoresis and visualized by ethidium bromide
The slides were then stained with 50 [micro]L ethidium bromide
(20[micro]g/mL; Sigma) and covered with a coverslip for image analysis.
RT-PCR products were subject to electrophoresis on an agarose gel, stained with ethidium bromide
, and visualized under UV light.