The resulting products were visualized under UV light in 2% agarose gel stained with ethidium bromide
with a 100-bp DNA ladder, and the sizes of bands were determined using the Syngene Gene Tool with the gel documentation system.
The resulting restriction fragments were separated by 2% agarose electrophoresis and visualised by ethidium bromide
staining and UV transillumination.
Internet or lab manual as an ethidium bromide
On show: Damien Hirst's Ethidium Bromide
The relative quantities of DNA migrating as either form were visualized using ethidium bromide
After electrophoresis the gel was stained with ethidium bromide
and the image captured with a digital camera (Kaiser Fototechnik GmbH & Co.
Dilute aqueous solutions of ethidium bromide
, a mutagen, are used to stain biological specimens.
The purification procedure removes ethidium bromide
, linkers, and labels.
After incubating for an hour, a sample of cells was removed from each well and treated with an acridine orange plus ethidium bromide
stain and with 1% paraformaldehyde.
5 micrograms per milliliter ([[micro]gram]/mL) of ethidium bromide
The most commonly used fluorescent dyes include DAPI, acridine orange, ethidium bromide
, and propidium iodide.
Visualisation is achieved by staining with ethidium bromide
(a fluorescent DNA intercalating dye) and viewing the gel under UV light (Figure 2).