ethidium bromide


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ethidium bromide

[e′thid·ē·əm ′brō‚mīd]
(organic chemistry)
C21H20BrN3 Dark red crystals with a melting point of 238-240°C; used in treating trypanosomiasis in animals and as an inhibitor of deoxyribonucleic and ribonucleic acid synthesis. Also known as homidium bromide.
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In the control culture, a greater reduction in the fluorescence between 10 and 20 minutes due to the higher extrusion of ethidium bromide by the NorA efflux pump was observed.
The amplified product (498 bp) was electrophoresed in 2% agarose gel stained with ethidium bromide (0.
The resulting products were visualized under UV light in 2% agarose gel stained with ethidium bromide with a 100-bp DNA ladder, and the sizes of bands were determined using the Syngene Gene Tool with the gel documentation system.
The resulting restriction fragments were separated by 2% agarose electrophoresis and visualised by ethidium bromide staining and UV transillumination.
The intercalating dye ethidium bromide, which had been used to demonstrate the concept of real-time PCR, was soon replaced with asymmetric cyanine dyes that were less inhibitory to the PCR (6).
Ethidium Bromide and Propidium Iodide are classic cell impermeant DNA stains that are structurally similar to phenanthridium intercalators.
They also required the students to use what they had learned to draw a picture of a gel under different circumstances and compare staining with ethidium bromide versus methylene blue.
ACTINOMYCIN D AND ETHIDIUM BROMIDE BOUND TO CALF THYMUS DNA.
The restriction enzyme digestion of all the PCR products, as visualized in Agarose gel electrophoresis after ethidium bromide staining.