In the control culture, a greater reduction in the fluorescence between 10 and 20 minutes due to the higher extrusion of ethidium bromide
by the NorA efflux pump was observed.
The amplified product (498 bp) was electrophoresed in 2% agarose gel stained with ethidium bromide
PCR product was analyzed in agarose gel stained with ethidium bromide
Efflux-mediated response of Staphylococcus aureus exposed to ethidium bromide
The resulting products were visualized under UV light in 2% agarose gel stained with ethidium bromide
with a 100-bp DNA ladder, and the sizes of bands were determined using the Syngene Gene Tool with the gel documentation system.
The resulting restriction fragments were separated by 2% agarose electrophoresis and visualised by ethidium bromide
staining and UV transillumination.
The intercalating dye ethidium bromide
, which had been used to demonstrate the concept of real-time PCR, was soon replaced with asymmetric cyanine dyes that were less inhibitory to the PCR (6).
and Propidium Iodide are classic cell impermeant DNA stains that are structurally similar to phenanthridium intercalators.
They also required the students to use what they had learned to draw a picture of a gel under different circumstances and compare staining with ethidium bromide
versus methylene blue.
ACTINOMYCIN D AND ETHIDIUM BROMIDE
BOUND TO CALF THYMUS DNA.
The restriction enzyme digestion of all the PCR products, as visualized in Agarose gel electrophoresis after ethidium bromide
Silver, commassie blue and ethidium bromide