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The following analytical standards were purchased from Sigma-Aldrich (Germany): ethyl butanoate, ethyl crotonate, butyl acetate, butyl butanoate, ethyl hexanoate, hexyl acetate, propyl hexanoate, ethyl octanoate, octyl acetate, isoamylhexanoate, methyl acetate, hexyl hexanoate, ethyl decanoate, citronellyl acetate, 2-heptenal, nonanal, trans-2-nonenal, decanal, cis-3-hexen-1-ol, trans-3-hexen-1-ol, hexan-1-ol, 1octanol, linalool, [alpha]-pinene, [beta]-pinene, myrcene, limonene, [gamma]-terpinene, [alpha]-terpinene, terpinolene, [alpha]-ionone, [beta]-ionone, 6methyl-5-hepten- 2-one, rose oxide, and citronellol.
The semiquantification was performed using calibration curves made of 5 concentration levels (0.2-60 [micro]g of standard per vial) of 10 representative compounds for the main volatile classes in passion fruit: ethyl butanoate, ethyl hexanoate, hexyl hexanoate, ethyl crotonate, hexan-1-ol, linalool, 6-methyl-5-hepten-2-one, limonene, [gamma]-terpinene, and decanal (Sigma-Aldrich).