Amplification of the eubacterial
16S rRNA gene was detected with template DNA from each insect, but no amplification products were detected in any of the insects based on the primers for Archaea, Fungi, Helicosporida, or Microsporidia.
Computer-assisted comparison of the determined sequences with all GenBank entries and in-house eubacterial
16S rDNA databank revealed scores of homology that led to a reliable list of candidates.
Genomic DNA was extracted from the pooled sample and 16S ribosomal DNA genes were amplified using universal eubacterial
These questions had been posed previously in reference to research conducted on leaderless mRNA in a different eubacterial
genus, and a list of sequenced wild type and mutant E.
Castaldini M, Turrini A, Sbrana C, Benedetti A, Marchionni M, Mocali S, Fabiani A, Landi S, Santomassimo F, Pietrangeli B, Nuti MP, Miclaus N, Giovannetti M (2005) Impact of Bt com on rhizospheric and soil eubacterial
communities and on beneficial mycorrhizal symbiosis in experimental microcosms.
Briefly, 16S rRNA gene fragment was amplified by the polymerase chain reaction (PCR) using the eubacterial
universal primers 27F (5'-AGAGTTTGATCCTGGC TCAG-3') and 1492R (5'-GGTTACCTTGTTACGACTT3').
Structure-specific endonucleolytic cleavage of nucleic acids by eubacterial
These are universal eubacterial
primers binding to phylogenetically highly conserved regions of the 16S rRNA gene.
1990) and 16S rDNA was amplified by using the universal eubacterial
primers such as forward primer, 5'-AGAGTTTGATCCTGGCTCAG-3' and reverse primer, 5'-ACGGCTACCTTGTTACGACTT-3' (Allen et al.
16S ribosomal RNA genes were amplified using universal eubacterial
primers, and PCR products were cloned and sequenced.
larvae, because these primer pair was universal eubacterial
primer that designed to classify related bacteria depending on sequencing results.
Polymerase chain reaction (PCR) amplification of the bacterial 16S gene was performed using the universal eubacterial
primer set 27f-1387r (27f: 5'-AGA GTT TGA TCM TGG CTC AG-3') and 1387r (5'-GGG CGG WGT GTA CAA GGC-3') (Marchesi et al.