exonuclease


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exonuclease

[¦ek·sō′nü·klē‚ās]
(biochemistry)
Any of a group of enzymes which catalyze hydrolysis of single nucleotide residues from the end of a deoxyribonucleic acid chain.
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References in periodicals archive ?
Characterization of the native and recombinant catalytic subunit of human DNA polymerase gamma: identification of residues critical for exonuclease activity and dideoxynucleotide sensitivity.
The Tentacle Probe assay used Taq TSP polymerase (exonuclease deficient) with a 2-min denaturation at 95 [degrees]C, followed by 45 cycles of 95 [degrees]C for 0 seconds, 60 [degrees]C for 10 seconds, and 70 [degrees]C for 10 seconds (mechanism in Fig.
PCR products were treated with 2 units shrimp alkaline phosphatase (Roche Diagnostics) and 5 units exonuclease I (USB) at 37 [degrees]C for 30 min followed by 99 [degrees]C for 15 min to inactivate the enzymes.
Therefore, we developed a simple 5-min method for rapid single-step selective digestion of the complementary strand with lambda exonuclease, which leads to increased hybridization signals and improved differentiation of single-nucleotide polymorphisms (SNPs) on DNA microarrays.
Ultrasensitive detection of protein using an aptamer-based exonuclease protection assay.
Subsequently, a genotype PCR assay for the MGL2-09 sample was performed by using 9 additional thermostable DNA polymerases either with 3'-5' exonuclease activity ("proofreading") or without 3'-5' exonuclease activity ("nonproofreading"), including KAPA2G Robust HotStart (KAPA Biosystems), Klentaq1 (DNA Polymerase Technology, St Louis, Missouri), AmpliTaq Gold (Thermo Fisher Scientific), Platinum Taq (Thermo Fisher Scientific), FailSafe PCR Enzyme Blend (Lucigen Corp, Middleton, Wisconsin), TaKaRa LA Taq (Takara Bio USA, Mountain View, California), Platinum Taq High Fidelity (Thermo Fisher Scientific), Q5 High-Fidelity (New England Biolabs, Ipswich, Massachusetts), and Deep Vent DNA Polymerase (New England Biolabs).
epsilon: Aphidicolin inhibition and the relationship between polymerase and exonuclease activity, Biochemistry, 32, 8568 (1993).
The degradation of the strand in a 5' [right arrow] 3' direction commences once the exonuclease is activated.
In order to test the ability of such bio-additives to produce a DNA-free surface, an acrylic latex paint was formulated with an exonuclease, Phosphodiesterase I (from Eastern Diamondback rattlesnake), capable of degrading both DNA and RNA polymer strands.
Oligouridylated pre-let-7 can also be degenerated by the 3'5' exonuclease Dis312 [23, 24].
For the high specificity, exonuclease ExoVII was used to digest nonhybrid probe, which could eliminate the influence of nonspecific adsorption on the determination results at maximum.