exonuclease


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exonuclease

[¦ek·sō′nü·klē‚ās]
(biochemistry)
Any of a group of enzymes which catalyze hydrolysis of single nucleotide residues from the end of a deoxyribonucleic acid chain.
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References in periodicals archive ?
To optimize lambda exonuclease reaction, 5 pg of the purified dsDNA was incubated with 5 U lambda exonuclease in a total reaction volume of 30 [micro]l in a reaction buffer at 37[degrees]C.
Nuclease P1 and S1 are single-strandspecific nucleases that degrade DNA or RNA either endonucleolytically or as a 3 k exonuclease.
1993) Yeast cells lacking 5' [right arrow] 3' exonuclease 1 contain mRNA species that are poly(A) deficient and partially lack the 5' cap structure.
Telomeres therefore serve to protect chromosomes and maintain genomic integrity from recombination, exonuclease degradation and end-to-end fusion (Blackburn et al.
4), who managed to develop the fundamental principle of the technique, by taking advantage of the 5' to 3' exonuclease activity of Taq polymerase enzyme.
10 [micro]l of PCR product was digested with Exonuclease I (10U) and Shrimp Alkaline Phosphatase (1U) for 15 min at 37[degrees]C prior to cycle sequencing to remove leftover primers and dNTPs.
Ontario, Canada) which exhibits 3'[right arrow]5' exonuclease (proof reading) activity according to manufacturer's instructions.
see Figure 1) utilizes the 5' exonuclease activity of Taq polymerase as originally described in 1991 by Holland et al.
The probe, which contains both a fluorescence reporter dye at the 5'-end (6-carboxyfluorescein, 6-FAM: maximum emission wavelength = 518 nm) and a quencher dye at the 3'-end (6-carboxytetramethyl rhodamine, TAMRA: maximum emission wavelength = 582 nm), is degraded by the 5'-3' exonuclease activity of the Taq DNA polymerase, and the resulting fluorescence is detected by a laser in the sequence detector (TaqMan ABI Prism 7700 Sequence Detector System; PerkinElmer).
Detection of specific polymerase chain reaction product by utilizing the 5' to 3' exonuclease activity of Thermus aquaticus DNA polymerase, Proceedings of the National Academy of Sciences of the USA (1991) 88:7276-7280.
Also, other mutagenization processes that can be used in combination with, or in lieu of, saturation mutagenesis, including, for example: (a) assembly and/or reassembly of polynucloetide building blocks (including sections of genes &/or of gene families) mediated by a source of exonuclease activity such as exonuclease III; and (b) introduction of two or more related polynucleotides into a suitable host cell such that a hybrid polynucleotide is generated by recombination and reductive reassortment.
Phosphate diesters can be cleaved by enzymes such as RNAse which is present in HIV reverse transcriptase and 3'-5' exonuclease from DNA polymerase I.