Single-stranded DNA is generated by using Lambda Exonuclease
Characterization of the native and recombinant catalytic subunit of human DNA polymerase gamma: identification of residues critical for exonuclease
activity and dideoxynucleotide sensitivity.
The Tentacle Probe assay used Taq TSP polymerase (exonuclease
deficient) with a 2-min denaturation at 95 [degrees]C, followed by 45 cycles of 95 [degrees]C for 0 seconds, 60 [degrees]C for 10 seconds, and 70 [degrees]C for 10 seconds (mechanism in Fig.
PCR products were treated with 2 units shrimp alkaline phosphatase (Roche Diagnostics) and 5 units exonuclease
I (USB) at 37 [degrees]C for 30 min followed by 99 [degrees]C for 15 min to inactivate the enzymes.
Therefore, we developed a simple 5-min method for rapid single-step selective digestion of the complementary strand with lambda exonuclease
, which leads to increased hybridization signals and improved differentiation of single-nucleotide polymorphisms (SNPs) on DNA microarrays.
Ultrasensitive detection of protein using an aptamer-based exonuclease
Subsequently, a genotype PCR assay for the MGL2-09 sample was performed by using 9 additional thermostable DNA polymerases either with 3'-5' exonuclease
activity ("proofreading") or without 3'-5' exonuclease
activity ("nonproofreading"), including KAPA2G Robust HotStart (KAPA Biosystems), Klentaq1 (DNA Polymerase Technology, St Louis, Missouri), AmpliTaq Gold (Thermo Fisher Scientific), Platinum Taq (Thermo Fisher Scientific), FailSafe PCR Enzyme Blend (Lucigen Corp, Middleton, Wisconsin), TaKaRa LA Taq (Takara Bio USA, Mountain View, California), Platinum Taq High Fidelity (Thermo Fisher Scientific), Q5 High-Fidelity (New England Biolabs, Ipswich, Massachusetts), and Deep Vent DNA Polymerase (New England Biolabs).
epsilon: Aphidicolin inhibition and the relationship between polymerase and exonuclease
activity, Biochemistry, 32, 8568 (1993).
The degradation of the strand in a 5' [right arrow] 3' direction commences once the exonuclease
In order to test the ability of such bio-additives to produce a DNA-free surface, an acrylic latex paint was formulated with an exonuclease
, Phosphodiesterase I (from Eastern Diamondback rattlesnake), capable of degrading both DNA and RNA polymer strands.
Oligouridylated pre-let-7 can also be degenerated by the 3'5' exonuclease
Dis312 [23, 24].
For the high specificity, exonuclease
ExoVII was used to digest nonhybrid probe, which could eliminate the influence of nonspecific adsorption on the determination results at maximum.