explant


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explant

[′eks‚plant]
(cell and molecular biology)
An excised fragment of a tissue or an organ used to start a cell culture.
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DP tissues were extirpated from tooth by decoronation as shown in Fig.1C and were cultured via outgrowth/tissue explant method.
Whereas, the maximum number of bulblets per explant (5.33) was found on a MS medium supplemented with 2.0 mg/l BAP (Fig.
These somatic embryos have directly formed from the inoculated explant (protocorm), thus characterizing their direct origin, since callus formation was not observed.
Regeneration of plants from transformed explants is difficult in genetic transformation protocols, and for recalcitrant species like common bean is one of the unsolved aspects (Liu, Park, Kanno, & Kameya, 2005; Amugune et al., 2011; Collado et al., 2015).
Figure 2 also shows data for explant length and average length of 'Pircinque' strawberry shoots, with the highest length of explants always being obtained in the MS culture medium, regardless of the time of immersion in the bioreactors and in the control treatment.
Kintzios & Michaelakis (1999) investigated callus induction and somatic embryogenesis of flower end tissue explants in MS medium containing 26.8 [micro]M NAA and 11.5 [micro]M Kinetin levels.
Of the 170 patients submitted to LT for HCC treatment between 1999 and 2016, six (3.5%) were identified with an isolate finding of SLF when the explant was analyzed.
(2008), the type of explant to be used (Jiang et al., 2012), conditions of the growing environment (Tokuji & Kuriyama, 2003), and the use of growth regulators (Jimenes, 2005; Lavanya et al., 2014) because these latter act significantly in the mitotic recovery of differentiated tissues, modifying the quiescent cellular metabolism in an active metabolism, being able to provide a large number of individuals (Quiroz-Figueroa, 2006; Hendrawati et al., 2012).
(2006) observed a maximum of 4.64 shoots per Pinus pinea explant.
Different protocols of surface sterilisation may vary with the type of chosen plant for culture and selected explants to use.
The results showed that various optimal parameters such as 100 mg L-1 of kanamycin concentration for selection of transformants, 2 d of pre-cultivation time, 100 umol L-1 of acetosyringone concentration, 15 min of infection time and 2 d of co-cultivation time were obtained using cotyledonary node explants. The rooting frequency observed on Murashige and Skoog (MS) medium supplemented with 0.2 mg L-1 indole acetic acid and 400 mg L-1 cefotaxime was found to be 100.00%.
There are different cryopreservation techniques, and the choice of which is most appropriate will depend on the explant to be cryopreserved (Sakai & Engelmann, 2007).