Total RNA was extracted from pretreated cells and explanted
by TRIpure reagents (RP1202, BioTeke, Beijing, China), and the concentration of RNA was quantified using ultraviolet spectroscopy (Nano-Drop 2000, Thermo, USA).
Growth and organogenesis in explanted
tissue of Amaryllis in culture.
Explanted tissues were transferred to three replicates of culture plates and examined under the microscope to detect viability of cells by observing the motility of their villi.
On days 1-7 of tissue culture, motility of explanted gill cells and growth of cells from the periphery of the explants were observed.
asinina cell culture, which yielded cell growth from the explanted tissues.
Pathologic examination of the explanted
donor liver demonstrated focal acute subcapsular necrosis, large droplet fat accumulation, and mild chronic portal inflammation.
However, shares of the firm's stock plummeted, from about $45 per share in April 2001 to $9.90 July 5, when the firm issued a press release admitting to receipt of a warning letter and 'additional information' from the CDC that showed that the explanted
heart valves had signs of fungal infection."
Colonies to be analyzed were explanted
onto fresh 12-mm glass coverslips and allowed to gro w until a stolon reached the edge of the coverslip, at which time shape analysis was begun.
The parents of our patient did not want any further surgery, so we explanted
the device and completed a radical mastoidectomy.
Forty (74.1%) of the explanted valves were bioprostheses, and 14 (25.9%) were allografts.
All pulmonary valve bioprostheses explanted from January 1995 to June 2003 at Toronto General Hospital have been included in the current study.
However, in subsequent communications, a CDC representative has informed CryoLife that based on certain additional information available to the CDC, which information CryoLife has requested but not yet been able to obtain, signs of fungal infection were isolated on a culture of the explanted