One month after introduction, establishment of up to 83.33% of explants
was observed for the treatment that combined fungicide with the lower concentration of mercuric chloride (T3) (Figure 1).
Three hours after the LPS or saline injections, all animals were euthanized by decapitation and collected anterior pituitaries were divided into 6 explants
and transferred immediately to 24-well plates (Becton Dickinson Labware, Franklin Lakes, NJ, USA).
Shoot tip and nodal explants
of Salvadora persica and Solanum suratensse were used as an explants
, collected from the experimental field of Plant Tissue Culture Laboratory, PCSIR Labs Complex Karachi.
It suggests that the genetic transformation system developed using cotyledonary node explants
is efficient and successful in cucumber.
During cryopreservation, the samples survival will depend on the glassy state of explants
, that is, the direct transition of water from liquid to an amorphous or glassy solid (Engelmann, 2011).
We aim to develop genetic transformation technology for commercial soybean varieties by identifying the most optimal way to regenerate shoots from different soybean explants
and evaluate whether shoots regeneration capacity is related to the maturity grouping of the soybean cultivars.
KEYWORDS: Dental pulp, Tissue explant
, Mesenchymal stem cells, Permanent teeth, Primary/Deciduous teeth, Tissue engineering.
Contaminations were notreported on bulb scale explants
Approximately 30 explants
showing physiological ages 80 and 120 DASI were inoculated in test tubes containing 10 mL of the aforementioned nutrient medium and kept in the dark in a growth room at 25 [+ or -] 2[degrees]C in order to assess the somatic embryogenesis-induction period.
The inocultion was performed by immersing the explants
in the bacterial suspension for 15 min under constant agitation (900 rpm), and bacterial suspension excess was eliminated with sterile towel paper.
Two independent studies were carried out, characterized by the stages of multiplication and rooting of 'Pircinque' strawberry explants
, both evaluated after 45 days.
Kintzios & Michaelakis (1999) investigated callus induction and somatic embryogenesis of flower end tissue explants
in MS medium containing 26.8 [micro]M NAA and 11.5 [micro]M Kinetin levels.