explant

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explant

[′eks‚plant]
(cell and molecular biology)
An excised fragment of a tissue or an organ used to start a cell culture.
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The dissected half embryos with or without cotyledon were chosen as explants to test their in vitro response.
It suggests that the genetic transformation system developed using cotyledonary node explants is efficient and successful in cucumber.
The experimental design was completely randomized, with 6 replicants of 5 explants per treatment.
This method can protect sensitive explants against harmful conditions, for example, the exposure to potentially toxic substances present in cryoprotectants used during the procedure (Subramaniam, Sinniah, Khoddamzadeh, Periasamy, & James, 2013).
Therefore, we thought that if the direct communication of all factors released from the subchondral bone, including the bone marrow, with the cartilage of osteochondral explants can be restricted to crosstalk via the cartilage bone interface, cartilage matrix composition may be maintained.
The explants were incubated at 22+3C, under a 16-hour photoperiod and in luminous intensity of 2000 lux.
This study was conducted to investigate the feasibility of using the leaf and petiole explants from the field plants for direct organogenesis of plant tissues.
Explants selected were leaf, stem, cotyledon, internode and apical bud.
Subsequently, the seeds were washed three times with sterile water before the explants were inoculated in WPM (LLOYD; MCCOWN, 1980) supplemented with 0.