Inclusion bodies were not observed in induced cells bearing the nonrecombinant expression vector
, which is predicted to generate a 4-kDa truncated peptide, but this species is not readily recovered and detected under these purification conditions.
For rapid drug development, ZYCOS has developed the BIOTOPE(R) DNA expression vector
and GENCAP(TM) DNA delivery system.
The pET-26b(+) expression vector
with kanamycin resistance gene was used for cloning and expression purpose.
To establish the transgene-expressing cell lines, each transgene expression vector
was co-transfected with piggyBac transposase using Lipofectamine 3000 (Invitrogen, USA) according to the manufacturer's protocol.
To induce recombination between loxP sites, the Cre recombinase expression vector
pCMV-Cre was transfected into the HeLa cell-integrated DNA cassette.
coli, the electroporation transformation of competent yeast cells needed to linearize the expression vector
After receiving of hpaA construction in PGH vector and confirmation by mentioned methods in material & method section, has been successfully sub-cloned into the expression vector
The GeneArt CRISPR Nuclease Vector with OFP Reporter is a similar expression vector
system featuring an orange fluorescent protein (OFP) reporter.
In the present study, LbCHI31 and LbCHI32 were, respectively, cloned into a prokaryotic expression vector
pET52b and transformed into E.
The collaboration aims to enhance clinical development services by integrating cDNA synthesis, cloning into mammalian expression vector
, transfection, clone selection and stable cell line development with cell banking, upstream/downstream process development and GMP manufacturing.
Murhammer (chemical and biochemical engineering, University of Iowa) details steps for techniques used in baculovirus and insect cell culture in this guide for biochemists, molecular biologists, and biochemical engineers using baculovirus expression vector
systems (BEVs) and/or insect cells for producing recombinant proteins.
Briefly, monkey kidney CV-1 cells (0.42 x [10.sup.6] cells/6-cm dish) were transfected using the calcium phosphate DNA precipitation method with the human androgen receptor expression vector
pCMVhAR (50 of 100 ng/dish) and the luciferase reporter rector under control of the mouse mammary tumor virus promoter (MMTV-luc, 5 [micro]g/dish).