Iron bacteria A was isolated from soil by using standard culture medium which consisted of the following: ferric ammonium citrate, magnesium sulfate, ferrous ammonium sulfate, dipotassium hydrogen phosphate, calcium chloride, and sodium nitrate, PH 6.8 to 7.0.
In addition, the absorption peak at 1386.3 [cm.sup.-1] belonging to the stretching vibration of -COOH was related to the existence of ferric ammonium citrate in the culture medium.
Strains were able to grow on glucose peptone agar, grow and pigmentation in ferric ammonium citrate
media, and were able to use 3 - ketolactose instead of mannitol in the culture medium.
haemophilum is a fastidious organism that requires media supplemented with ferric ions in the form of heroin, hemoglobin, or ferric ammonium citrate
, and incubation at 30[degrees]C-32[degrees]C for several weeks.
Both OPSP and TSC combine sodium metabisulphite & ferric ammonium citrate
to form the basis of the indicator system with which C.
The FDA list of iron compounds generally recognized as safe (GRAS) includes elemental iron, ferrous ascorbate, ferrous carbonate, ferrous citrate, ferrous fumarate, ferrous gluconate, ferrous lactate, ferrous sulfate, ferric ammonium citrate
, ferric chloride, ferric citrate, ferric pyrophosphate and ferric sulfate.
haemophilum will grow on egg- or agar-based media only if supplemented with 0.4% hemoglobin, 60 [mu]M hemin, or 15 mg/mL ferric ammonium citrate
Although the chemicals are not classified as toxic, ferric ammonium citrate
is considered an irritant if the powder comes in contact with the skin; potassium ferricyanide is classified as harmful.
The ferric ammonium citrate
(FAC) component is provided as a supplement to be added after sterilisation.
haemophilum is unique among Mycobacterium species owing to its special growth requirements: it grows best at 30[degrees]C and requires an iron supplement (hemin or ferric ammonium citrate
With lab M's new Fraser BrothPLUS and Fraser BrothPLUS, the antibiotic supplement is blended into the base medium, but the ferric ammonium citrate
(FAC) component is supplied separately as a supplement.