Aguas, "The use of osmium tetroxide-potassium ferrocyanide
as an extracellular tracer in electron microscopy.," Stain Technology, vol.
The reducing power (RP) of a compound may serve as a significant indicator of its potential antioxidant capacity to reduce the potassium ferricyanide ([Fe.sup.3+]), to form potassium ferrocyanide
These authors revealed that upon sunlight exposure, the conversion of ferricyanide to ferrocyanide
and the reverse reaction depended strongly on the pH of the solution whether or not the Ti[O.sub.2] catalyst was present.
This assay was used to convert the potassium ferricyanide complex to potassium ferrocyanide
complex, which would constitute ferrous complex by reducing ferric chloride .
After washing with PBS, cells were incubated at 37[degrees]C with freshly prepared senescence-associated SA-[beta]-Gal staining solution: 1 mg/ml 5-bromo-4-chloro-3-indolyl P3-D-galactoside (X-Gal), 40 mM citric acid/sodium phosphate, pH 6.0, 5 mM potassium ferrocyanide
, 5 mM potassium ferricyanide, 150 mM NaCl, and 2 mM MgCl2.
After that, cells were incubated overnight at 37[degrees]C without C[O.sub.2] in a freshly prepared staining buffer (1 mg/ml X-gal, 40 mM citric acid/sodium phosphate, pH 6.0, 5 mM potassium ferrocyanide
, 5mM potassium ferricyanide, 150 mM NaCl, and 2 mM Mg[Cl.sub.2]).
Sections were washed three times in PBS and incubated in freshly senescence-associated [beta]-galactosidase (SA-[beta]-gal) staining solution (1 mg/mL X-gal, 40 mM citric acid/sodium phosphate (pH 6.0), 5 mM potassium ferricyanide, 5 mM potassium ferrocyanide
, 150 mM NaCl, and 2 mM Mg[Cl.sub.2]) for 14 h at 37[degrees]C without C[O.sub.2].
GUS staining of rice calli and leaves was carried out using 5-bromo-4-chloro-3-indolyl glucuronide (X-Gluc) solution (0.1 M NaP[O.sub.4], 0.1% Triton X-100, 0.5 mM potassium ferrocyanide
, 0.5 mM potassium ferricyanide, 2 mM X-Gluc, and 10 mM EDTA pH 7.0) according to Jefferson et al.
In addition, sulfur retention ability of Bayer red mud from an alumina plant was improved by fusel salt and waste mother liquor of sodium ferrocyanide
as the main sulfur fixation agent and the calcium based natural mineral materials as servicing additives.
Sodium dihydrogen phosphate (NaH2PO4), potassium ferrocyanide
([K.sub.3]Fe[(CN).sub.6]), trichloroacetic acid (TCA), iron-III-chloride (Fe[Cl.sub.3]), potassium peroxydisulfate ([K.sub.2][O.sub.8][S.sub.2]) copper-II-chloride (Cu[Cl.sub.2]), sodium acetate (NaCH3COO), hydrochloric acid (HCl), tris, iron-II-sulfate (FeS[O.sub.4]), iron-II-chloride (Fe[Cl.sub.2]), disodium hydrogen phosphate ([Na.sub.2]HP[O.sub.4]), methionine, ethanol, ethylene-diaminetetraacetic acid (EDTA), ammonium thiocyanate (N[H.sub.4]SCN), sodium bicarbonate (NaHC[O.sub.3]), sodium hydroxide (NaOH), disodium sulfate ([Na.sub.2]S[O.sub.4]), sodium perchlorate (NaCl[O.sub.4]), and disodium carbonate ([Na.sub.2]C[O.sub.3]) were obtained from Merck (Merck, made in Germany).
The supernatant collected was assayed for ferrocyanide
content using 4, 7-diphenyl-1, 10-phenanthroline disulfonic acid disodium salt, absorption was recorded at 535 nm ([member of] = 20,500 [M.sup.-1] [cm.sup.-1]).
The post fixing was performed with 1% osmium tetroxide and 0.8% potassium ferrocyanide
. After the cells were dehydrated, it were submitted to critical point drying C[O.sub.2] and metallization with gold.