FCS:
fetal calf serum; T: times of recovery after vincristine removal (0, 6, and 18 h).
Effect of different volumes of Glasgow minimum essential medium (GMEM) with 5%
fetal calf serum (FCS) was monitored on growth of BHK-21 cells in roller culture bottles.
enzyme-linked immunosorbent assay; FCS,
fetal calf serum; FDA, Food and Drug Administration; [IC.sub.50], minimal concentration required to inhibit 50% cytopathic effect; IFN, interferon; HEp-, human larynx epidermoid carcinoma cell; MEM.
For immunofluorescence studies, the OC exptants were fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 5% Triton X-100 in PBS with 10%
fetal calf serum.
Cells were cultured in DMEM containing 25 mmol/L glucose and 200 mL/L heat-inactivated
fetal calf serum and grown at 37 [degrees]C in air containing 5% [CO.sub.2*].
Briefly, 0.5 ml of heparinized blood samples were obtained from two healthy females donors and were placed in a sterile culture bottle containing 7 ml of RPMI 1640 medium, supplemented with 1.5 ml of
fetal calf serum, 0.1 ml of phytohaemagglutinin and 0.1 ml of antibiotic-antimycotic mixture and were placed in an incubator at 37[degrees]C for 24 hr.
The needs of the
fetal calf will not have an impact on the cow's nutrient needs during the first trimester.
A team of Japanese scientists has successfully extracted a previously undiscovered substance from
fetal calf serum which can protect neurons of the central nervous system (CNS) from harmful endogenous materials.
All postmortem trauma indicated by the bones of two adult cows and a
fetal calf was documented prior to student excavation.
Typically,
fetal calf serum is added during various steps of the islet isolation process to inactivate enzymes, as well as to provide nutrients.
The support medium that was tested at first included vitamins A and C (1 nM), L-phosphatidycholine (0.1 mg/ml), glucose (0.01%), 5% bovine serum albumen, 5%
fetal calf serum, cofactors NAD and Co-A (1 nM) and 5 [[micro]meter] concentrations of ATP and GTP.
On the other side was a special mixture of
fetal calf serum that acted as an attractant to the neutrophils.
