FCS: fetal calf
serum; T: times of recovery after vincristine removal (0, 6, and 18 h).
Effect of different volumes of Glasgow minimum essential medium (GMEM) with 5% fetal calf
serum (FCS) was monitored on growth of BHK-21 cells in roller culture bottles.
enzyme-linked immunosorbent assay; FCS, fetal calf
serum; FDA, Food and Drug Administration; [IC.sub.50], minimal concentration required to inhibit 50% cytopathic effect; IFN, interferon; HEp-, human larynx epidermoid carcinoma cell; MEM.
For immunofluorescence studies, the OC exptants were fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 5% Triton X-100 in PBS with 10% fetal calf
Cells were cultured in DMEM containing 25 mmol/L glucose and 200 mL/L heat-inactivated fetal calf
serum and grown at 37 [degrees]C in air containing 5% [CO.sub.2*].
Briefly, 0.5 ml of heparinized blood samples were obtained from two healthy females donors and were placed in a sterile culture bottle containing 7 ml of RPMI 1640 medium, supplemented with 1.5 ml of fetal calf
serum, 0.1 ml of phytohaemagglutinin and 0.1 ml of antibiotic-antimycotic mixture and were placed in an incubator at 37[degrees]C for 24 hr.
The needs of the fetal calf
will not have an impact on the cow's nutrient needs during the first trimester.
A team of Japanese scientists has successfully extracted a previously undiscovered substance from fetal calf
serum which can protect neurons of the central nervous system (CNS) from harmful endogenous materials.
All postmortem trauma indicated by the bones of two adult cows and a fetal calf
was documented prior to student excavation.
Typically, fetal calf
serum is added during various steps of the islet isolation process to inactivate enzymes, as well as to provide nutrients.
The support medium that was tested at first included vitamins A and C (1 nM), L-phosphatidycholine (0.1 mg/ml), glucose (0.01%), 5% bovine serum albumen, 5% fetal calf
serum, cofactors NAD and Co-A (1 nM) and 5 [[micro]meter] concentrations of ATP and GTP.
On the other side was a special mixture of fetal calf
serum that acted as an attractant to the neutrophils.