Polymorphous low grade adenocarcinoma: glial fibrillary
acidic protein staining in the differential diagnosis with cellular mixed tumors.
Immunohistochemical evaluation of CN suggests that synaptophysin consistently stains the neuropil, especially in fibrillary
zones and perivascular cell-free areas.
4-8) Type C melanocytes showed identical staining pattern when compared with type A and type B melanocytes using S100 protein, Leu-7, glial fibrillary
acid protein, and myelin-basic protein.
Rare cases may stain positively for desmin and glial fibrillary
acidic protein, but not typically for leukocyte common antigen or actin.
On immunohistochemistry, the immature cells stained positively for synaptophysin but not for chromogranin, glial fibrillary
acid protein (GFAP), and S- 100 protein.
5,8) Chordomas will stain positive with monoclonal staining for S-100 and EMA, but negative for glial fibrillary
acidic protein, high-molecular-weight keratin, and muscle-specific actin and desmin.
Immunohistochemical staining of angiocentric gliomas is typically positive with antibodies to glial fibrillary
acidic protein, S-100 protein, and vimentin.
Immunostaining was performed with glial fibrillary
acidic protein, neurofilament protein, S-100, and the proliferation index MIB-1 (Ki-67).
A recent study found that expression of glial fibrillary
acidic protein and CD57 could be reliably used in cell block preparations to differentiate pleomorphic adenoma from ACC.
Masson's strichrome staining or an S-100 protein and glial fibrillary
acidic protein (GFAP) are most helpful in accentuating the neural tissue in the background fibrosis (figure 2).
Stains for CK20, uroplakin III, prostate-specific antigen, prostatic acid phosphatase, vimentin, S100, smooth muscle actin, chromogranin, synaptophysin, and glial fibrillary
acidic protein were negative.
Immunohistochemistry for S-100 protein (dilution: 1:100) (figure 3) and neuron-specific enolase (dilution: 1:50) showed strong positivity in the tumor cells but was negative for epithelial membrane antigen (dilution: 1:50) and glial fibrillary
acid protein (dilution: 1:5,000).