Results from all bioassays were subjected to probit analysis to determine the [LC.sub.50] and [LD.sub.50] values, fiducial limits
, and slopes (Alarie 1988; Sakuma 1998).
Treatments###LC50 (Fiducial Limits
bassiana y = 0.327x + 2.207 0.0448 (B1 strain) Entomopathogenic [LC.sub.50] Fiducial limits
fungi (ppm) Lower limit L.
Baits [LC.sub.50] (conidia per mL) (95% Fiducial limits
) BMet + UFP * 2.1 x [10.sup.6] (7.3 x 105-6.1 x [10.sup.6]) BMet + Bcon ** 3 x [10.sup.6] (1.4 x [10.sup.6]-6.3 x [10.sup.6]) Baits [LC.sub.90] (conidia per mL) (95% Fiducial limits
) BMet + UFP * 3.2 x [10.sup.7] (1 x [10.sup.7]-3.2 x [10.sup.8]) BMet + Bcon ** 7.3 x [10.sup.7] (2.9 x [10.sup.7]-3.1 x [10.sup.8]) * Bait with Metarhizium conidia and untreated filter paper.
L[C.sub.50] and L[C.sub.90] values along with their fiducial limits
, regression equation and chi-square were calculated.
maculatus 5.72 (4.69-6.62)1 95% lower and upper fiducial limits
are shown in parenthesis
The use of confidence or fiducial limits
illustrated in the case of the binomial.
The LC50 values (and 95% fiducial limits
) ranged from 0.06 (0.02-0.26) to 0.80 (0.26-2.46) ng/[micro]L for each insecticide tested.
b 95% fiducial limits
estimated using SPSS10.0 Institute (2000).
quinquefasciatus 138 275.4 (135.9-140) (273-277.8) * All values are in ppm; Figures in parentheses are 95% fiducial limits
The concentrations causing 50% ([LC.sub.50]) and 95% response ([LC.sub.95]) and the 95% fiducial limits
were determined by probit analysis using PoloPC (LeOra software 1987).
at 95% interval were used for differentiation of significance levels (Litchfield and Wilcoxon, 1949).