Among the topics are filamentous bacteriophage
structure and biology, leveraging synthetic phage-antibody libraries for panning on the mammalian cell surface, high-throughput and high-content screening using peptides, antibody humanization and affinity maturation using phage display, and native antibody libraries from natural repertoires.
Marks, "Targeted gene delivery to mammalian cells by filamentous bacteriophage
," Journal of Molecular Biology, vol.
Although other display systems have been described, the most popular vehicle for display remains the filamentous bacteriophage
. The nonlytic filamentous phage, like M13, infects strains of E.
Integration of the DNA of a novel filamentous bacteriophage
VSK from Vibrio cholerae 0139 into the host chromosomal DNA.
A filamentous bacteriophage
(CTX[PHI]) integrated in the Vibrio cholerae chromosome encodes the cholera toxin, and Matthew Waldor (New England Medical Center, Boston, MA) described another phage (RS1) that flanks the CTX[PHI] prophage in the bacterial chromosome and is important for the CTX[PHI] prophage propagation.
Centrum Voor Plantenveredelings-en (Wageningen, Netherlands) has patented phagemid vectors comprising i) a promoter operatively linked to a gene coding for a translational fusion between a peptide and a filamentous single strand DNA bacteriophage coat protein or a part thereof, which promoter is induced by expression of gene IV of a filamentous bacteriophage
, ii) a replication origin derived from a filamentous single strand DNA bacteriophage, and iii) a plasmid replication origin.