fluorescent in situ hybridization

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fluorescent in situ hybridization

[flə¦res·ənt in¦sit‚chü ‚hī·brə·də′zā·shən]
(genetics)
A technique in which a deoxyribonucleic acid (DNA) probe is labeled with a fluorescent dye (that can be visualized under a fluorescent microscope) and then hybridized with target DNA, usually chromosome preparations on a microscopic slide. It is used to precisely map genes to a specific region of a chromosome in prepared karyotype, or can enumerate chromosomes, or can detect chromosomal deletions, translocations, or gene amplifications in cancer cells. Abbreviated FISH.
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Determination of HER2/neu Status: A Pilot Study Comparing HER2/neu Dual In Situ Hybridization DNA Probe Cocktail Assay Performed on Cell Blocks to Immunohistochemisty and Fluorescence In Situ Hybridization Performed on Histologic Specimens.
inhibitor assays) Body fluid evaluation Bone marrow prep or evaluation Budgeting/inventory control/purchasing Cytochemical stains Flow cytometry Hemoglobinopathy evaluation Method evaluation Personnel management Platelet function studies PT/PTT mixing studies CORRELATING SCOPE OF PRACTICE WITH MOLECULAR BIOLOGY EXAM Cytogeneticist DNA/RNA extraction Fluorescence In Situ Hybridization (FISH) Microscopy (signal analysis) Array-CGH Medical Laboratory Scientist DNA/RNA extraction Nucleic Acid Testing (e.
KEY WORDS: top shell, chromosome, karyotype, telomere, Turbo cornutus, Mollusca, invertebrate, fluorescence in situ hybridization
Metaphase and interphase fluorescence in situ hybridization mapping of rice genome with bacterial artificial chromosomes.
The international Molecular Cytogenetics market is driven by a number of factors, including the increasing use of molecular cytogenetic tools, such as Fluorescence In Situ Hybridization (FISH) and Array-based Comparative Genomic Hybridization (aCGH) for analyzing complex disease traits, gene mapping, gene discovery, and for improving molecular diagnostics capabilities.
Fluorescence in situ hybridization is a morphology-based method and the best results can be achieved when involving cytopathologists and cytotechnicians with an expertise both in morphology and FISH evaluation.
Comparison of dual-color dual-hapten brightfield in situ hybridization (DDISH) and fluorescence in situ hybridization in breast cancer HER2 assessment.
9 May 2013 - Italian Cancer Genetics (CGI) said today it has entered into a partnership with Nikon Instruments to distribute on the Italian market its DNA probes for cancer diagnostics based on fluorescence in situ hybridization (FISH) .
According to the company, HER2 IQFISH pharmDx, which is a fluorescence in situ hybridization (FISH) assay, uses the Dako IQISH technology, which is based on its fast IQISH hybridization buffer chemistry.
Like IHC, fluorescence in situ hybridization (FISH)-based detection of the ERBB2 [v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian); also known as HER2] gene copy number is a morphologydriven slide-based assay that features DNA hybridization with fluorescently labeled probes (9).
ABSTRACT Chromosomal location of the major ribosomal RNA genes (rRNA) were studied in the dwarf surfclam (Mulinia lateralis, Say) using fluorescence in situ hybridization (FISH).
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