immunoassay

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Related to fluorescence polarization immunoassay: Cloned Enzyme Donor Immunoassay

Immunoassay

An assay that quantifies antigen or antibody by immunochemical means. The antigen can be a relatively simple substance such as a drug, or a complex one such as a protein or a virus. See Antibody, Antigen

The reactants are first mixed so that a varying quantity of one (A) is added to a constant amount of the other (B). The formation of an immune (antigen-antibody) complex is measured as a function of the varied reactant (A). The result is represented by a “standard curve” for reactant A. An unknown sample is tested by adding it to reactant B. The extent of the measured change is referred to the standard curve, and thereby is obtained the amount of reactant A which produces a comparable change. The amount is represented as the content of reactant A in the unknown sample. See Immunofluorescence, Immunology, Radioimmunoassay

McGraw-Hill Concise Encyclopedia of Bioscience. © 2002 by The McGraw-Hill Companies, Inc.

immunoassay

[‚im·yə·nō′a‚sāy]
(immunology)
A laboratory detection method that uses antibodies to react with specific substances.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
References in periodicals archive ?
1 h 3 h Asian ginseng 1 0.79 0.51 2 0.71 0.47 3 0.74 0.49 American ginseng 1 0.68 0.49 2 0.58 0.55 3 0.61 0.44 Indian ginseng 1 0.60 0.30 2 0.62 0.25 3 0.51 0.20 * Measured by the fluorescence polarization immunoassay. Table 2.
Enzyme-multiplied immunoassay (EMIT 2000) digoxin assay compared with fluorescence polarization immunoassay and Amerlex [sup.125]I-RIA at two Australian centers.
tHcy was measured by fluorescence polarization immunoassay (9).
Comparison with the fluorescence polarization immunoassay on an 1Mx analyzer (Abbott) showed the following results: [y.sub.1Mx]=0.878[x.sub.HPLC] + 0.583; r = 0.878; n = 136.
The metabolite 3-hydroxy-MEGX, which cross-reacts in the fluorescence polarization immunoassay, is a minor metabolite in humans (11), but it can make a major contribution to lidocaine metabolites in some animal models (12).
This last technique was then extended to ELISA (11) and fluorescence polarization immunoassay (12).
The Abbott IMx[R] homocysteine assay (Abbott Laboratories) is a fully automated, fluorescence polarization immunoassay. This assay involves the initial reduction of Hcy, mixed-disulfide, and protein-bound Hcy to free Hcy with dithiothreitol, followed by conversion to S-adenosyl-L-homocysteine (SAH) by bovine SAH hydrolase and excess adenosine.

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