positive positive DFAT: direct fluorescent antibody
test; bact.: bacteriological; ep: epididymis; F1 to F4: fetal organs.
Comparative evaluation of the rabies fluorescent antibody
test and direct microscopic examination at the Research Institute for Tropical Medicine.
Atanasiu, "The fluorescent antibody
test," in Laboratory Techniques in Rabies, F.-X.
Immuofluorescence staining: The slides were deparaffinized with xylene, alcohol and with fluorescent antibody
ABBREVIATIONS: AFB = acid-fast bacilli; CDC = Centers for Disease Control and Prevention; CSF = cerebrospinal fluid; DFA = direct fluorescent antibody
; DNA = deoxyribonucleic acid; ELISA = enzyme-linked immunosorbent assay; FITC = fluorescein isothiocyanate; GIS = Geographical Information System; H1N1 = influenza A virus; LRN = Laboratory Response Network; MDS = Molecular Detection Services; MGIT = mycobacteria growth indicator tube; MTB = Mycobacterium tuberculosis; PCR = polymerase chain reaction; PFGE = pulsed field gel electrophoresis; RNA = ribonucleic acid; STEC = shiga-toxin producing Escherichia coli; TB = tuberculosis; WHO = World Health Organization; WNV = West Nile virus
To find out whether Hv1 was the correct channel in sperm, the researchers added a green fluorescent antibody
stain to the channel and noted that Hv1 was abundant.
Use of a blue-excitable (Alexa 488) or a green-excitable (Alexa 555) fluorescent antibody
with a red-excitable one (Alexa 633) will provide complete spectral separation and accurate quantitation of both antibodies on one blot.
Direct fluorescent antibody
testing for respiratory viruses was positive in eight flocked swab samples and three nasopharyngeal aspirates.
techniques use the properties of dyes such as fluorescein and rhodamine to emit visible light in response to ultraviolet radiation (Figure 2-15).
Several different serological techniques have been proposed: complement-fixation test , fluorescent antibody
[18,26], capillary tube agglutination test , rapid card agglutination test , indirect fluorescent antibody
test [10,17] and enzyme linked immunosorbent assay (ELISA) [11,25,30,32,33].
The results indicated that VZV-specific IgM was negative [< 1:20; Merifluor VZV IgM indirect fluorescent antibody
, (IFA); Meridian Bioscience Inc., Cincinnati, OH, USA], but the VZV IgG was positive (1:80 on the basis of > 1:10, Merifluor VZV IgG IFA), with high sensitivity (93.9% for IgM and 100% for IgG) and high specificity (100% for both antibodies) according to the manufacturer's instructions.