examined (%) group, y Bartolo Ivamirapinta Microscopy IFAT Microscopy IFAT and PCR and PCR 1-10 2/29 (6.9) 3/9 (33.3) 1/21(4.7) 5/6 (83.3) 11-20 1/16 (6.2) 1/3 (33.3) 0/42 (0) 3/10 (30) 21-30 1/23 (4.3) 7/7 (100) 1/15 (6.6) 1/3 (33.3) 31-40 0/19 (0) 3/4 (75) 1/21 (4.7) 2/3 (66.6) 41-50 0/11 (0) 0/2 (0) 0/13 (0) 3/4 (75) 51-60 1/11 (9.0) 2/8 (25) 0/13 (0) 2/6 (33) 61-70 0/10 (0) 5/10 (50.0) 1/15 (6.6) 4/11 (36.4) >70 0/4 (0) 1/4 (25) 0/8 (0) 1/4 (25) Total 5/123 (4.1) 22/47 (46.8) 4/148 (2.7) 21/47 (44.7) * IFAT, indirect
fluorescent antibody test.
Indirect
Fluorescent Antibody Test: Serial dilutions of serum samples (1:40, 1:80, 1:160, 1:320, 1:640 and 1:1,280) were prepared in phosphate buffered saline (PBS) and 20ul of each dilution was transferred to Lyme antigen coated wells on a slide (Fuller Laboratories, Fullerton, CA).
Serum was tested by using an indirect
fluorescent antibody test with human lung cancer cells (CRL-5803; American Type Culture Collection) for detection of antibodies against SVA (B.
[UKPRwire, Thu May 02 2019] Direct
fluorescent antibody test kits detects the existence of an antigen (particularly a protein on the surface of a bacteria, virus or any other microbe).
[ClickPress, Fri Apr 26 2019] Rapid visualization of bacteria from a sample such as a throat swab or sputum is achieved through direct
fluorescent antibody test which drive the market of direct
fluorescent antibody test kits.
During 2000-2013, samples from 468 animals showing clinical signs of rabies or to whom humans were exposed were submitted to the CVL for rabies testing by using the
fluorescent antibody test (5).
Samples were tested by the
fluorescent antibody test, rabies tissue culture infection test, real-time reverse transcription PCR, and heminested reverse transcription PCR, as described (6,7).
On November 12, CDC reported detecting no rabies virus antigens in the skin biopsy by direct
fluorescent antibody test, nor amplicons in the saliva or skin biopsy specimens by reverse transcription--polymerase chain reaction.
We developed an indirect
fluorescent antibody test (IFAT) for primary testing of human serum.
