fluorescent in situ hybridization


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fluorescent in situ hybridization

[flə¦res·ənt in¦sit‚chü ‚hī·brə·də′zā·shən]
(genetics)
A technique in which a deoxyribonucleic acid (DNA) probe is labeled with a fluorescent dye (that can be visualized under a fluorescent microscope) and then hybridized with target DNA, usually chromosome preparations on a microscopic slide. It is used to precisely map genes to a specific region of a chromosome in prepared karyotype, or can enumerate chromosomes, or can detect chromosomal deletions, translocations, or gene amplifications in cancer cells. Abbreviated FISH.
References in periodicals archive ?
Rapid diagnostic of bacterial meningitis by real-time PCR and fluorescent in situ hybridization.
Advantages of peptide nucleic acid for sensitive site directed 16S rRNA fluorescent in situ hybridization (FISH) detection of Campylobacter jejuni, Campylobacter coli, and Campylobacter lari.
6,7) Diagnosis is confirmed by fluorescent in situ hybridization (FISH) examination with a chromosome 22 library.
It also utilises conventional Fluorescent in Situ Hybridization (FISH) methods to detect large deletions, amplifications and translocations in nine different chromosomal loci.
Researchers using a variety of cytogenetic microarray platforms can now quickly identify aberrations via Nexus and immediately identify/link to available oligonucleotide-based fluorescent in situ hybridization (FISH) probes for follow-up studies.
A relatively new technique called comparative genomic hybridization may soon replace fluorescent in situ hybridization as the method of choice for obtaining preimplantation genetic diagnoses for aneuploidy in blastomeres.
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