When the sputum samples turn out to be negative after ZN or fluorescent staining
, the diagnosis of TB rests upon nonspecific tests like x-ray chest in resource limited settings.
The cornea stayed stable (g) with a negative fluorescent staining
(h) at the eighth-month visit.
(b) DiI-ac-LDL and FITC-UEA-1 double fluorescent staining
However fluorescent staining
is technically more complicated.3
Regardless the type of treatment, sperm volume, progressive motility, vigor, sperm morphology, hyposmotic-swelling test and fluorescent staining
did not change significantly during the evaluated periods and maintained in the normal range described for the canine species (P>0.05).
Photomicrographs of the soleus muscle of the animals in groups [IF.sub.(3)], [IS.sub.(3)], [IF.sub.(10)], and [IS.sub.(10)], A-L: Longitudinal sections immunolabeled with rabbit polyclonal anti-dystrophin (green fluorescence) and mouse monoclonal [ED1] anti-CD68 (red fluorescence) antibodies, nuclear fluorescent staining
defined by DAPI (blue fluorescence).
Although it is very convenient to detect the sperm motility by CASA, few reports detecting sperm motility by fluorescent staining
techniques are available.
Direct fluorescent staining
makes it possible to detect cells before they become visible to the human eye using digital imaging and earlier results minimize warehousing space, reduce personnel time and allow direct intervention in manufacturing.
Among their topicsare the light microscope, image capture, the confocal microscope,aberrations and their consequences, deconvolution and imageprocessing, stereoscopy and reconstruction for three-dimensionalimaging, fluorescent staining
, evanescent wave microscopy, and beyondthe diffraction limit.
Crystal violet staining and fluorescent staining
for CD31, f-actin and nuclei were performed according to protocols described previously (6).
In brief, fluorescent staining
was performed using monoclonal antibodies (Becton Dickinson, Biosciences, USA) against cell surface receptors [anti-human CR1 (clone E11; WS No.: III 204), CD55 (clone IA10; WS No.: V BP352, S031) and CD59 (clone p282 (H19); WS No.: V S006)].
Simultaneous fluorescent staining
of R bands and specific heterochromatic regions (DA/DAPI) in human chromosomes.