Fluorochrome


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Fluorochrome

 

a substance used in fluorescence, or luminescence, microscopy to study objects that do not possess a natural capacity to fluoresce. When fluorochromes are introduced into an organism, they are adsorbed by the cells, which consequently acquire the capacity to fluoresce. Fluorochromes may be dyes (auramine, coryphosphin), pigments and their derivatives (chlorophyll, porphyrin), or alkaloids (berberine). Fluorescence in microscopic objects stained with fluorochromes is brought about by ultraviolet, violet, or blue light. Fluorescence microscopy with the use of fluorochromes brings out structural details more clearly than does ordinary staining, especially in the case of biological specimens. Because the fluorescence microscopy method is highly sensitive, the fluorochrome concentration can be very low and consequently living organisms can be observed and their metabolic processes studied.

REFERENCES

Levshin, V. L. Fotoliuminestsentsiia zhidkikh i tverdykh veshchestv. Moscow-Leningrad, 1951.
Zelenin, A. V. Liuminestsentnaia tsitokhimiia nukleinovykh kislot. Moscow, 1967.
References in periodicals archive ?
The fifth chapter on lasers, fluorochromes, and filters is particularly well done.
Both instrument settings were appropriate for simultaneous measurement of beads and cells stained with the respective fluorochromes.
These instruments are capable of conducting five-color analysis from either single or dual laser excitation, and are equipped with a collinear second laser that maintains a stable alignment with a single beam path and allows greater versatility in antibody and fluorochrome choices.
Successful CGH was indicated by (1) the presence of 4',6'-diamidino-2-phenylindole (DAPI)-stained centromere regions, indicating adequate blocking by cot-1 DNA, (2) high fluorescence intensity for both fluorochromes, (3) high signal-to-noise ratio, (4) homogeneous appearance of the fluorescence without granularity, and (5) dark background of the image.
It is important to realize that the fluorochromes vary in their stability, so it is important to label the RNAs both ways; that is, a reciprocal hybridization should be performed in which the Cy3-labeled RNA is labeled with Cy5 and the results compared.
Cells are roughly spherical with a size of 1-2 [micro]m and contain no distinct nucleus, as determined by epifluorescence microscopy using the nucleicacid fluorochrome acridine orange.
Even the thickness of the smear affects the activity of the disinfectant; however, staining by either the Ziehl-Neelsen or fluorochrome method effectively inactivates M.
DNA content was determined using the fluorochrome bisben-zimidazole (Hoechst 33258) after cell lysis by freezing with water (Rago et al.
The use of detection antibodies labeled with the fluorochrome R-phycoerythrin allows quantification of antigen-antibody reactions that occur on the microsphere surface by measurement of the relative fluorescence intensity.
SMFT uses a small aliquot of uncentrifuged urine and an equivalent amount of fluorochrome solution (acridine orange in a sterile phosphate buffer solution) on a slide.
14,15] The laboratory findings characteristic of an acute meningitis, negative fluorochrome smears of CSE and CT findings interpreted as suggestive of CNS toxoplasmosis led to failure to consider tuberculous meningitis in the differential diagnosis until later in the patient's course in several cases in this series.
Use of fluorochrome calcein as an in situ growth marker in the brown mussel Perna perna.