Fluorochrome


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Fluorochrome

 

a substance used in fluorescence, or luminescence, microscopy to study objects that do not possess a natural capacity to fluoresce. When fluorochromes are introduced into an organism, they are adsorbed by the cells, which consequently acquire the capacity to fluoresce. Fluorochromes may be dyes (auramine, coryphosphin), pigments and their derivatives (chlorophyll, porphyrin), or alkaloids (berberine). Fluorescence in microscopic objects stained with fluorochromes is brought about by ultraviolet, violet, or blue light. Fluorescence microscopy with the use of fluorochromes brings out structural details more clearly than does ordinary staining, especially in the case of biological specimens. Because the fluorescence microscopy method is highly sensitive, the fluorochrome concentration can be very low and consequently living organisms can be observed and their metabolic processes studied.

REFERENCES

Levshin, V. L. Fotoliuminestsentsiia zhidkikh i tverdykh veshchestv. Moscow-Leningrad, 1951.
Zelenin, A. V. Liuminestsentnaia tsitokhimiia nukleinovykh kislot. Moscow, 1967.
References in periodicals archive ?
Fluorochrome specific beads rather than beads hard-dyed with fluorochrome surrogates provide a better basis for quantitative setup.
3] fluorochrome on the fourth pair of chromosomes was observed in all 3 Atta species; a secondary constriction was also visible, indicating that this GC rich heterochromatic region probably corresponds to the nucleolus organizer region (NOR).
Their compatibility with other popular fluorochromes and fluorescent protein combinations offers versatility that will further expand researchers' options for multi-color panels.
This system includes the use of small single strand DNA sequences of approximately 20-25 nucleotides, labeled with a fluorochrome donor and a fluorochrome receptor (quencher).
Abstract--This study documents validation of vertebral band-pair formation in spotted gully shark (Triakis megalopterus) with the use of fluorochrome injection and tagging of captive and wild sharks over a 21-year period.
An assessment of the effects of instrumentation, monoclonal antibody and fluorochrome on flow-cytometric immunophenotyping has suggested that by controlling these parameters the inter-laboratory agreement on CD4+ T cell percentages will greatly improve (21).
16) The principle behind the broth is the fluorochrome is suppressed in the presence of oxygen however, once the free oxygen is utilized by the organism and carbon dioxide is generated, the fluorochrome is released and detected under ultraviolet light.
LEDs of different colors can be used in combination, giving users the option of seeing multiple fluorochromes simultaneously or rapidly capturing sequential images of each fluorochrome.
The comparison of the two different fluorochrome banding patterns showed that [CMA.
Bioaerosol characterization by flow cytometry with fluorochrome.
Ziehl-Neelsen (ZN) stain) or fluorochrome dyes (auramine stain) and examined with light and fluorescent microscopy, respectively, as shown in Fig.