Fluorochrome

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Fluorochrome

 

a substance used in fluorescence, or luminescence, microscopy to study objects that do not possess a natural capacity to fluoresce. When fluorochromes are introduced into an organism, they are adsorbed by the cells, which consequently acquire the capacity to fluoresce. Fluorochromes may be dyes (auramine, coryphosphin), pigments and their derivatives (chlorophyll, porphyrin), or alkaloids (berberine). Fluorescence in microscopic objects stained with fluorochromes is brought about by ultraviolet, violet, or blue light. Fluorescence microscopy with the use of fluorochromes brings out structural details more clearly than does ordinary staining, especially in the case of biological specimens. Because the fluorescence microscopy method is highly sensitive, the fluorochrome concentration can be very low and consequently living organisms can be observed and their metabolic processes studied.

REFERENCES

Levshin, V. L. Fotoliuminestsentsiia zhidkikh i tverdykh veshchestv. Moscow-Leningrad, 1951.
Zelenin, A. V. Liuminestsentnaia tsitokhimiia nukleinovykh kislot. Moscow, 1967.
References in periodicals archive ?
When the probes are hybridized, the two fluorochromes are close.
In the excitation process, photons from excitation source propagate to fluorochromes. Subsequently, fluorescent photons emitted from fluorochromes propagate to detectors in the emission process.
Those results later were confirmed using the same PLA nanoparticles loaded with the hydrophilic fluorochrome 4-Di-2-Asp, and with the lipophilic fluorochrome Bodipy 630/ 650.
The GC and AT rich regions were detected using the fluorochromes Chromomycin [A.sub.3] ([CMA.sub.3]) and 4'6-diamidin-2-phenylindole (DAPI) according to Schweizer (1980).
Furthermore, the TB exclusion assay by flow cytometry means that simultaneous cell staining with monoclonal antibodies conjugated with fluorochromes detectable on the FL2 (585/42 nm) or FL1 (530/30 nm) channel is possible.
Politz, "Use of caged fluorochromes to track macromolecular movement in living cells," Trends in Cell Biology, vol.
This technique is based on the intersection of the microorganisms and / or microcapsules with an argon laser at 488 nm, that generates fluorescence which enable to distinguish between live microorganisms and damaged microorganisms due to the reactivity of the fluorochromes. The samples were previously incubated in the dark for a time of 20 minutes in presence of fluorochromes (SYTO[R]9 and propidium iodide) in a ratio (1:1).
Developed for use on the company's Gallios and Navios series flow cytometers and compatible with all proprietary fluorochromes, the kits enable a clear focus on the cytoplasmic and nuclear compartments of the cell.
* Compensation: Compensation is the process by which the spectral overlap between different fluorochromes is mathematically eliminated.