Fluorochrome

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Fluorochrome

 

a substance used in fluorescence, or luminescence, microscopy to study objects that do not possess a natural capacity to fluoresce. When fluorochromes are introduced into an organism, they are adsorbed by the cells, which consequently acquire the capacity to fluoresce. Fluorochromes may be dyes (auramine, coryphosphin), pigments and their derivatives (chlorophyll, porphyrin), or alkaloids (berberine). Fluorescence in microscopic objects stained with fluorochromes is brought about by ultraviolet, violet, or blue light. Fluorescence microscopy with the use of fluorochromes brings out structural details more clearly than does ordinary staining, especially in the case of biological specimens. Because the fluorescence microscopy method is highly sensitive, the fluorochrome concentration can be very low and consequently living organisms can be observed and their metabolic processes studied.

REFERENCES

Levshin, V. L. Fotoliuminestsentsiia zhidkikh i tverdykh veshchestv. Moscow-Leningrad, 1951.
Zelenin, A. V. Liuminestsentnaia tsitokhimiia nukleinovykh kislot. Moscow, 1967.
References in periodicals archive ?
14) Using unique monoclonal antibodies that are highly specific for certain cellular elements and covalently labeled with various fluorochromes that emit at different wavelengths, flow cytometry becomes a powerful diagnostic tool.
The DNA then needs to be isolated and purified from the tumor sample, labeled with a fluorochrome, and hybridized.
Assessment of the effects of instrumentation, monoclonal antibody, and fluorochrome on flow cytometric immunophenotyping: a report based on 2 years of the NIAID DAIDS flow cytometry quality assessment program.
The most basic requirement of a fluorescence microscopy light source is closely matching the excitation wavelength of the fluorochrome to achieve a high contrast image - that is, an image with a high signal-to-noise ratio.
Brenner's team applied dyes known as fluorochromes to a single pair of matched chromosomes from each of more than 100 cells from each volunteer.
The fluorescence probes used in examining probiotic viability are fluorochromes designed to express membrane integrity, membrane potential or the enzyme activity of the cell.
The host antibodies can be isolated, purified, and tagged with fluorochromes - color-coded molecules that can be detected visually.
The Fluoro-Jade Dyes: Novel, Sensitive, and Reliable Fluorochromes for the Histochemical Localization of Degenerating Neurons.
The system must contain superresolucnE[degrees] module based on stochastic localization of different fluorochromes (SMLM) and at least one other super-module and resolution based on stimulated emission depletion (STED) and / or on the basis of structured excitation (SIM).
Diploid metaphases of Pachycondyla harpax submitted to sequential staining with fluorochromes CMA3/ DAPI a) Ilheus, Bahia; b) Barrolandia, Belmonte, Bahia; c, d) Vera Cruz district, southwest of Una Biological Reserve.