Fluorochrome

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Fluorochrome

 

a substance used in fluorescence, or luminescence, microscopy to study objects that do not possess a natural capacity to fluoresce. When fluorochromes are introduced into an organism, they are adsorbed by the cells, which consequently acquire the capacity to fluoresce. Fluorochromes may be dyes (auramine, coryphosphin), pigments and their derivatives (chlorophyll, porphyrin), or alkaloids (berberine). Fluorescence in microscopic objects stained with fluorochromes is brought about by ultraviolet, violet, or blue light. Fluorescence microscopy with the use of fluorochromes brings out structural details more clearly than does ordinary staining, especially in the case of biological specimens. Because the fluorescence microscopy method is highly sensitive, the fluorochrome concentration can be very low and consequently living organisms can be observed and their metabolic processes studied.

REFERENCES

Levshin, V. L. Fotoliuminestsentsiia zhidkikh i tverdykh veshchestv. Moscow-Leningrad, 1951.
Zelenin, A. V. Liuminestsentnaia tsitokhimiia nukleinovykh kislot. Moscow, 1967.
References in periodicals archive ?
Fluorochrome specific beads rather than beads hard-dyed with fluorochrome surrogates provide a better basis for quantitative setup.
14) Using unique monoclonal antibodies that are highly specific for certain cellular elements and covalently labeled with various fluorochromes that emit at different wavelengths, flow cytometry becomes a powerful diagnostic tool.
Use of the fluorochrome calcein as an in situ growth marker in the brown mussel Perna perna.
iCyt), a subsidiary of Sony Corporation of America, has introduced a new line of 405nm-excited fluorochrome conjugated antibodies for flow cytometry.
An assessment of the effects of instrumentation, monoclonal antibody and fluorochrome on flow-cytometric immunophenotyping has suggested that by controlling these parameters the inter-laboratory agreement on CD4+ T cell percentages will greatly improve (21).
LEDs of different colors can be used in combination, giving users the option of seeing multiple fluorochromes simultaneously or rapidly capturing sequential images of each fluorochrome.
Common procedure involves the use of different fluorochromes able to differentiate DNA regions based on their nucleotide composition.
Additionally, for detection of the AT- and GC- rich chromatin regions, some chromosomal preparations were stained with the fluorochromes 4'-6-diamidino-2-phenylindole (DAPI) and chromomycin [A.
The excitation beams of an argon/krypton laser (488 and 568 nm) and band-pass emission filters (522/35 and 605/32 nm) were sequentially used for selective detection of the green (FITC) and red (Texas red) fluorochromes respectively.
The disk and optical components are all UV compatible and offer performance from 350 to 700 nm, making it suitable for most available fluorochromes.