fluorogenic substrate

fluorogenic substrate

[′flu̇r·ə‚jen·ik ′səb‚strāt]
(chemistry)
A nonfluorescent material that is acted upon by an enzyme to produce a fluorescent compound.
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Determination of neuraminidase kinetic constants using whole influenza virus preparations and correction for spectroscopic interference by a fluorogenic substrate. PLoS One.
The SensoLyte[R] Rh110 Matriptase Assay Kit provides a convenient assay for screening of Matriptase inhibitors and activators or for continuous assay of Matriptase activity using a highly sensitive fluorogenic substrate. Upon Matriptase cleavage, this substrate generates the Rh110 (Rhodamine 110) fluorophore which has a bright green fluorescence and can be detected at excitation/emission=496 nm/520 nm.
(1989) Preparation and application of a fluorogenic substrate for endo-[beta]-xylosidase.
They measured plasma PONl activity using a fluorogenic substrate and blood concentrations of mercury and selenium by inductively coupled plasma mass spectrometry.
An intramolecularly quenched fluorescent tripeptide as a fluorogenic substrate of angiotensin-I-converting enzyme and of bacterial dipeptidyl carboxypeptidase.
In this assay, a purified MMP-2 enzyme is incubated in the presence of a fluorogenic substrate with and without CAST.
Moreover, after injection of cRNA encoding [beta]-galactosidase, the levels of enzyme activity were measured using a fluorogenic substrate, revealing a peak of [beta]-galactosidase activity at 6 to 9 days; this activity was still detectable for at least 10 days after injection.
Later on, the chromogenic substrate was replaced by a fluorogenic substrate and this modification was of paramount importance.
The SensoLyte[R] 520 HDAC Activity Assay Kit provides a convenient, two-step homogeneous procedure for measuring HDAC activity using a cell-permeable fluorogenic substrate. Without a time-consuming cell extraction step, this assay can be used to measure HDAC activity directly in cell culture in a 96-well plate.
Cell lysates containing 50 [micro]g of protein were incubated with a caspase-3 fluorogenic substrate (DEVD-AFC), or a caspase-9 fluorogenic substrate (LEHD-AFC), for 1 h at 37[degrees]C.
NI assays are also easily adaptable to automation, particularly newer ones that use a chemiluminescent sialic acid substrate instead of a fluorogenic substrate (31).
This novel method relates to the use of a thin matrix that contains blood in the interstices of its structure and the use of a rhodamine-based fluorogenic substrate that has a high quantum yield and is not consumed significantly during the time of the assay.