The SensoLyte[R] Rh110 Matriptase Assay Kit provides a convenient assay for screening of Matriptase inhibitors and activators or for continuous assay of Matriptase activity using a highly sensitive fluorogenic substrate
This novel method relates to the use of a thin matrix that contains blood in the interstices of its structure and the use of a rhodamine-based fluorogenic substrate
that has a high quantum yield and is not consumed significantly during the time of the assay.
They measured plasma PONl activity using a fluorogenic substrate
and blood concentrations of mercury and selenium by inductively coupled plasma mass spectrometry.
An intramolecularly quenched fluorescent tripeptide as a fluorogenic substrate
of angiotensin-I-converting enzyme and of bacterial dipeptidyl carboxypeptidase.
Pharmaleads, a French biotech specialised in the custom design of substrates and inhibitors for all types of proteases announces the availability of its new FLUOFAST(R) PROTEASE technology for the rapid identification of a fluorogenic substrate
for a given PROTEASE from a large proprietary substrate library.
NI assays are also easily adaptable to automation, particularly newer ones that use a chemiluminescent sialic acid substrate instead of a fluorogenic substrate
The SensoLyte[R] 520 HDAC Activity Assay Kit provides a convenient, two-step homogeneous procedure for measuring HDAC activity using a cell-permeable fluorogenic substrate
Similarly, for Fabry disease, extracts from 3-mm punches were tested for GLA activity using an artificial fluorogenic substrate
(4-MU-[alpha]-gal) in the presence of an inhibitor, GalNac, for blocking the activity of [alpha]-Nacetylgalactosaminidase at acidic pH (4.
Cell lysates containing 50 [micro]g of protein were incubated with a caspase-3 fluorogenic substrate
(DEVD-AFC), or a caspase-9 fluorogenic substrate
(LEHD-AFC), for 1 h at 37[degrees]C.
These trapped beads are then exposed to a fluorogenic substrate
, which causes fluorescence to be generated in the immediate vicinity of the beads that bear enzyme-labeled complexes, thus creating countable pinpoints of light.
By using a fluorogenic substrate
, the TGA produces thrombin-generation curves in a fully automated manner that may be useful and sensitive enough to screen for either hypercoagulable states or hemorrhagic diatheses.
A hexapeptide with a sequence of His-Ser-Ser-Lys-Leu-Gln (HSSKLQ) was the most efficient fluorogenic substrate
for PSA to cleave without compromising specificity.