Later on, the chromogenic substrate was replaced by a fluorogenic substrate
and this modification was of paramount importance.
The SensoLyte[R] Rh110 Matriptase Assay Kit provides a convenient assay for screening of Matriptase inhibitors and activators or for continuous assay of Matriptase activity using a highly sensitive fluorogenic substrate
1989) Preparation and application of a fluorogenic substrate
They measured plasma PONl activity using a fluorogenic substrate
and blood concentrations of mercury and selenium by inductively coupled plasma mass spectrometry.
An intramolecularly quenched fluorescent tripeptide as a fluorogenic substrate
of angiotensin-I-converting enzyme and of bacterial dipeptidyl carboxypeptidase.
NI assays are also easily adaptable to automation, particularly newer ones that use a chemiluminescent sialic acid substrate instead of a fluorogenic substrate
In this assay, a purified MMP-2 enzyme is incubated in the presence of a fluorogenic substrate
with and without CAST.
Moreover, after injection of cRNA encoding [beta]-galactosidase, the levels of enzyme activity were measured using a fluorogenic substrate
, revealing a peak of [beta]-galactosidase activity at 6 to 9 days; this activity was still detectable for at least 10 days after injection.
Van Diggelen and coworkers have shown that this type of mutation can be revealed if the assay with the fluorogenic substrate
is carried out in the presence and absence of sphingomyelin; the wildtype enzyme shows stronger inhibition by the latter than the mutant enzyme (38).
The SensoLyte[R] 520 HDAC Activity Assay Kit provides a convenient, two-step homogeneous procedure for measuring HDAC activity using a cell-permeable fluorogenic substrate
Cell lysates containing 50 [micro]g of protein were incubated with a caspase-3 fluorogenic substrate
(DEVD-AFC), or a caspase-9 fluorogenic substrate
(LEHD-AFC), for 1 h at 37[degrees]C.
This novel method relates to the use of a thin matrix that contains blood in the interstices of its structure and the use of a rhodamine-based fluorogenic substrate
that has a high quantum yield and is not consumed significantly during the time of the assay.