Determination of neuraminidase kinetic constants using whole influenza virus preparations and correction for spectroscopic interference by a fluorogenic substrate
. PLoS One.
The SensoLyte[R] Rh110 Matriptase Assay Kit provides a convenient assay for screening of Matriptase inhibitors and activators or for continuous assay of Matriptase activity using a highly sensitive fluorogenic substrate
. Upon Matriptase cleavage, this substrate generates the Rh110 (Rhodamine 110) fluorophore which has a bright green fluorescence and can be detected at excitation/emission=496 nm/520 nm.
(1989) Preparation and application of a fluorogenic substrate
They measured plasma PONl activity using a fluorogenic substrate
and blood concentrations of mercury and selenium by inductively coupled plasma mass spectrometry.
An intramolecularly quenched fluorescent tripeptide as a fluorogenic substrate
of angiotensin-I-converting enzyme and of bacterial dipeptidyl carboxypeptidase.
In this assay, a purified MMP-2 enzyme is incubated in the presence of a fluorogenic substrate
with and without CAST.
Moreover, after injection of cRNA encoding [beta]-galactosidase, the levels of enzyme activity were measured using a fluorogenic substrate
, revealing a peak of [beta]-galactosidase activity at 6 to 9 days; this activity was still detectable for at least 10 days after injection.
Later on, the chromogenic substrate was replaced by a fluorogenic substrate
and this modification was of paramount importance.
The SensoLyte[R] 520 HDAC Activity Assay Kit provides a convenient, two-step homogeneous procedure for measuring HDAC activity using a cell-permeable fluorogenic substrate
. Without a time-consuming cell extraction step, this assay can be used to measure HDAC activity directly in cell culture in a 96-well plate.
Cell lysates containing 50 [micro]g of protein were incubated with a caspase-3 fluorogenic substrate
(DEVD-AFC), or a caspase-9 fluorogenic substrate
(LEHD-AFC), for 1 h at 37[degrees]C.
NI assays are also easily adaptable to automation, particularly newer ones that use a chemiluminescent sialic acid substrate instead of a fluorogenic substrate
This novel method relates to the use of a thin matrix that contains blood in the interstices of its structure and the use of a rhodamine-based fluorogenic substrate
that has a high quantum yield and is not consumed significantly during the time of the assay.