fluorogenic substrate

fluorogenic substrate

[′flu̇r·ə‚jen·ik ′səb‚strāt]
(chemistry)
A nonfluorescent material that is acted upon by an enzyme to produce a fluorescent compound.
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The SensoLyte[R] Rh110 Matriptase Assay Kit provides a convenient assay for screening of Matriptase inhibitors and activators or for continuous assay of Matriptase activity using a highly sensitive fluorogenic substrate.
This novel method relates to the use of a thin matrix that contains blood in the interstices of its structure and the use of a rhodamine-based fluorogenic substrate that has a high quantum yield and is not consumed significantly during the time of the assay.
They measured plasma PONl activity using a fluorogenic substrate and blood concentrations of mercury and selenium by inductively coupled plasma mass spectrometry.
An intramolecularly quenched fluorescent tripeptide as a fluorogenic substrate of angiotensin-I-converting enzyme and of bacterial dipeptidyl carboxypeptidase.
Pharmaleads, a French biotech specialised in the custom design of substrates and inhibitors for all types of proteases announces the availability of its new FLUOFAST(R) PROTEASE technology for the rapid identification of a fluorogenic substrate for a given PROTEASE from a large proprietary substrate library.
NI assays are also easily adaptable to automation, particularly newer ones that use a chemiluminescent sialic acid substrate instead of a fluorogenic substrate (31).
The SensoLyte[R] 520 HDAC Activity Assay Kit provides a convenient, two-step homogeneous procedure for measuring HDAC activity using a cell-permeable fluorogenic substrate.
Similarly, for Fabry disease, extracts from 3-mm punches were tested for GLA activity using an artificial fluorogenic substrate (4-MU-[alpha]-gal) in the presence of an inhibitor, GalNac, for blocking the activity of [alpha]-Nacetylgalactosaminidase at acidic pH (4.
Cell lysates containing 50 [micro]g of protein were incubated with a caspase-3 fluorogenic substrate (DEVD-AFC), or a caspase-9 fluorogenic substrate (LEHD-AFC), for 1 h at 37[degrees]C.
These trapped beads are then exposed to a fluorogenic substrate, which causes fluorescence to be generated in the immediate vicinity of the beads that bear enzyme-labeled complexes, thus creating countable pinpoints of light.
By using a fluorogenic substrate, the TGA produces thrombin-generation curves in a fully automated manner that may be useful and sensitive enough to screen for either hypercoagulable states or hemorrhagic diatheses.
A hexapeptide with a sequence of His-Ser-Ser-Lys-Leu-Gln (HSSKLQ) was the most efficient fluorogenic substrate for PSA to cleave without compromising specificity.