To 5 mg of F-21-HS, 200 [micro]L of dimethyl formamide
, 200 [micro]L of dioxan, and 100 [micro]L of distilled water containing 10 mg of N-hydroxysuccinimide and 20 mg of 1-ethyl-3-(3-dimethyl-amino-propyl) carbodiimide-HCL were added; the reaction mixture was vortex-mixed and kept overnight at 4 [degrees]C.
Results: The results of the research will provide data for mapping structure-activity relationships among novel formamide
Table 2 lists the contact angles between double-distilled water, ethylene glycol, glycerol or formamide
and poly(amic acid) and polyimide samples both, before and after plasma treatment.
(5b) a mixture of chloroform/alcohol with weight ratio 3:1, (5c) mixture of chloroform/tetrachloromethane with weight ratio 3:1, and (5d) mixture of chloroform/N,N-dimethyl formamide
(DMF) with weight ratio 3:1.
The multiplex samples were prepared for analysis on the ABI genetic analyzer by adding 2 [micro]L of the post-PER to 9 [micro]L of formamide
. Multiplex analysis performed on the ABI 310 capillary electrophoresis unit detected five wild-type samples (Fig.
peroxide (DCP, the initiator) and dimethyl formamide
(DMF, the inhibitor) supplied by Fluka Co.
The DNA fragments were diluted 10-fold (with HEX-labeled products) or 20-fold (with 6-FAM- or TET-labeled products) and were then mixed with formamide
and GS-500 TAMRA size standard (Applied Biosystems).
The NCO-terminated PU with various macroglycol diols (PTMG 2000, PBA 2000) was prepared in dimethyl formamide
Recently, we developed an accelerated procedure for the synthesis of formamides
. It was important to investigate if the procedure can be successfully applied for the synthesis of 1-phenylethylformamides with electron-donating substituents, for example N-[1-(4-methoxyphenyl) ethyl] formamide
Aliquots (25 [micro]L) of the denatured, neutralized amplicon were then combined with 100 [micro]L of hybridization solution [1.5X standard saline citrate (1X = 0.15 mol/L NaCI and 0.015 mol/L sodium citrate), 1 g/L sodium dodecyl sulfate, and 150 mL/L deionized formamide
in 0.5 mol/L sodium phosphate buffer, pH 4.2] in each of four wells coated with the two probes, in duplicate.
A 100-ng sample of control or tumor DNA was amplified in the presence of 0.4 [micro]mol/L each oligonucleotide primer and 1 U of Taq polymerase (Cetus-Perkin-Elmer) in a final volume of 25 [micro]L of the following solution: 200 [micro]mol/L each dNTP (Promega), 1.5 mmol/L Mg[Cl.sub.2], 50 mL/L deionized formamide
(Sigma), 0.166 mmol/L ([NH.sub.4])S[O.sub.4], 67 mmol/L Tris-HCl, pH 8.8, 0.1 mL/L Tween-20.