Unlike above discussed studies, in current study, the antioxidant activities of methanol extracts obtained from two different extraction methods were examined using by the three different methods (DPPH, ABTS, FRAP
Table-3: Effect of extracting conditions on the DPPH, FRAP
and [beta]-carotene antioxidant assays of SFE extracts
Table 1--Total phenolics, total flavonoids, inhibitory capacity ([IC.sub.50]), iron reduction capacity (FRAP
) and oxygen radical absorption capacity (ORAC), of extracts obtained by different extraction methods and at different temperatures.
The estimated values of MDA were significantly higher in smokers with COPD, but the levels of FRAP
were lower in the same group in comparison to non-smokers and smokers without COPD.
Antioxidant activities of each extract by DPPH, lipid peroxidation inhibitory, and FRAP
assays are shown in Table 4.
Fasting venous blood samples were collected through aseptic venipuncture into heparinized tubes and EDTA-containing tubes that were centrifuged (1500 x g, 10 min) to yield plasma for thiobarbituric acid reactive species (TBARS), ABTS, and FRAP
Boosted Regression Trees (BRT) analysis was used to examine the relative contribution of each polyphenolic compound to the antioxidant capacity (DPPH and FRAP
The antioxidant activity of yellow pigment was studied by measuring the FRAP
assay, superoxide radical scavenging activity ([O.sub.2.sup.-]), and ABTS assay.
The antioxidant activity was tested using four in vitro assays including, lipid peroxidation inhibition, scavenging effect on DPPH and ABTS radicals, and FRAP
One-factor analysis of variance (ANOVA) with Welch test and Games-Howell post hoc tests were used for FRAP
and initial weight as homogeneity of variance was not reached despite transformations.