freeze-fracture electron microscopy
Also found in: Acronyms.
freeze-fracture electron microscopy[¦frēz‚frak·chər i‚lek‚trän mi′kräs·kə·pē]
(cell and molecular biology)
A technique used to visualize the inside of cellular membranes. Rapidly frozen cells are ruptured (fractured) so as to split open the membrane and expose the interior surfaces. A thin layer of carbon together with a metal (usually platinum) is then evaporated over the specimen to produce a replica of the surface, which is removed and examined in the electron microscope.