To calculate number of egg masses, roots were washed with clean water and stained in 0.1% acid fuchsine
solution (acid fuchsine
dissolved in a 1:1:1 mixture of glycerol, lactic acid and distilled water) for 1-3 min.
To better distinguish the bacterial substances from crystal, the microorganism rendered in pink using basic fuchsine
, mixture of sand particles, and cementation solution, initial and stabilised crystals are defined by means of different characteristic values (RGB (243, 22, and 250); RGB (255, 255, and 255); RGB (10, 251, and 249); RGB (0, 20, and 250)), which corresponds to pink, white, light blue, and dark blue, respectively.
Semithin sections were stained with Epoxy Tissue Stain (EMS, USA), containing toluidine blue and basic fuchsine
. Ultrathin sections were mounted on formvar coated grids and double stained with 0.5% uranyl acetate and 3% lead citrate.
Then samples were stained for 5 minutes with Mallory red containing 100% acetic acid, fuchsine
acid, and Orange G (Merck, Darmstadt, Germany), then for 15 minutes in 1% molybdatophosphoric acid hydrate (VWR, Darmstadt, Germany), and then for 5 minutes in acid light green.
Qualitative examinations were performed via light microscopy on stained sections (1% methylene blue and 0.3% basic fuchsine
It might consist of a combination of different triphenyl-methane dyes such as fuchsine
and crystal violet, solvents and mordants like ethanol, acetone and lugol's iodine as reagents of Gram staining as the most extensively performed test in microbiology.
The slide-mounting process was as follows: (a) after making a small incision on the back of papaya mealybug, it was heated in 10% NaOH for 10 min; (b) the body contents was expelled and the body was gently flattened with a microspatula; (c) the preparation was stained morphologically in a saturated solution of fuchsine
in a mixture of distilled water, lactic acid and glycerol (1:1:1) for 3-5 h; (d) washed in glacial acetic acid for 1 h to stabilize the staining, transferred to lavender oil, allowed to stand for at least 1 h, and (f) placed in a drop of Canada balsam on a slide and covered with a cover glass.
Transversal freehand sections were made at middle third of the blade, followed by staining with basic fuchsine
diluted to 0.5% in ethanol 50% and Astra blue, to show the lignified walls and the cellulose walls in the leaf cells.
Teeth from both groups were then immersed in 2% basic fuchsine
dye for 8 hours separately.
Dissolve 1 gr of basic fuchsine
in 200 mL of distilled water and heat to 80[degrees]C, let it cool down, and add 2 gr of sodium or potassium metabisulfite, mix, and incorporate 10 mL of concentrated hydrochloric acid or 2.5 mL of 1N.
Slides were stained with toluidine blue and acid fuchsine
and were analyzed using a microscope connected to a video camera interfaced to a computer, where specific processing software was used for measurements (ImageJ 1.34, National Institutes of Health, Bethesda, MA, USA).