gDNA


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gDNA

(cell and molecular biology)
References in periodicals archive ?
RT-PCR Amplification of Bax, Bcl-2 and [beta]-Actin Gene: RNA extracted from small intestine tissue was reverse-transcribed into cDNA according to PrimeScript RT reagent Kit With gDNA Eraser instruction manual, and the objective band was observed after PCR amplification.
The first-strand cDNA was synthesized using primescript RT reagent kit with gDNA eraser from 2 pg total RNA after removing genome DNA.
The specificity against KRAS WT sequences was shown by including 16 000 copies (50 ng) of KRAS WT gDNA (K562 cell line DNA).
PCR products from Actin, ACP1, KASU, D9SD, FAD2-1 and FAD2-2 genes of Jatropha cinerea were sequenced from gDNA to obtain the greatest coverage of these genes and thus get more information of each one as possible.
We isolated genomic DNA (gDNA) from tomato leaves and embedded it in the silicone matrix.
RNA was extracted from testes tissues using the RNAeasy Plus Mini Kit (Qiagen, Germany), including a gDNA Eliminator column to avoid DNase digestion and a RNeasy Mini Spin columns to purify RNA samples.
We subjected extracted genomic DNA (gDNA) to a nested PCR.
A negative control was included in each experiment to ensure that there was no contamination by genomic DNA (gDNA).
Genomic DNA (gDNA) was precipitated by adding 5 mL 7.5 M ammonium acetate and 25 mL ice-cold ethanol (95-100%).
The genomic DNA (gDNA) was isolated from cultivable spore-forming bacteria by boiling suspended soil samples in 10 ml distilled water at 90[degrees]C for 30 min to kill all the vegetative cells and enrichment of the bacteria in Bushnell-Haas media (BH media) containing 1% heavy crude oil.
cDNA was synthesized using SuperScript[TM] RT reagent kit with gDNA Eraser (Takara, Shiga, Japan) from 1 ug RNA.
For the extraction of genomic DNA (gDNA), strain 56AF was grown in nutrient broth medium (Difco Laboratories, USA) at 25[degrees]C with shaking at 150 rpm for 24 h.