gene targeting


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gene targeting

[′jēn ‚tär·gəd·iŋ]
(genetics)
Replacement, by genetic engineering and homologous recombination, of a mutant (or wild-type) gene by a wild-type (or mutant) copy that may also contain a reporter gene.
References in periodicals archive ?
To this end, the student will genetically modify stem cells with transposition and gene targeting, develop methods to derive myoblasts from mouse and human pluripotent stem cells and magnetically purify the cells.
John Schoggins, Assistant Professor of Microbiology, used the cutting-edge CRISPR technology to identify the IFI6 gene as a potent antiviral gene targeting flaviviruses.
Kay 's contributions to the field of gene therapy also include development of viral and non-viral (minicircle) gene delivery systems as well as a novel gene editing approach for safe and effective targeted gene therapy; early success in rAAV-mediated treatment of hemophilia A and B, congenital blindness, and spinal muscular atrophy; and advancing RNAi and non-coding RNA biology for effective gene targeting strategies.
However, previously reported transgene was simply the random integration of donor cell, where rhLF has been reported to express in goats with hLF mono-allelic targeted into BLG locus by two round step of gene targeting, and the expression level of rhLF in the milk was 3.2 mg/mL.
"We also want to understand exactly how the inhibitor proteins block Cas9's gene targeting abilities, and continue the search for more and better CRISPR inhibitors in other bacteria," Rauch said.
Altogen Labs is unique in that it offers a comprehensive host of RNAi services (among many other preclinical biology lab services) including RNAi gene targeting, siRNA synthesis, functional in vitro validation via qPCR and Western Blot, siRNA encapsulation, in vivo siRNA protection and tissue-targeted delivery.
The companys scientific officer Anna Messer comments: Compared to other gene targeting technologies, our TALENs kits offer researchers more ease in use and great efficiency.
"If a patient tests positive for EGFR then they can be treated differently using gene targeting therapy called anti-EGFR which will only target the affected gene.
The new patent further strengthens Hamilton Thome's IP position, and advances the field of microscopy with innovative technology that can be used in intricate research applications such as stem cell research and gene targeting.
The precise gene targeting and high efficiency of Sigma's ZFN technology allows KBLB to build on its proof of concept research and the resulting transgenic silkworm is expected to be capable of spinning pure spider silk at commercially viable production levels.
"Such gene targeting in a probiotic bacteria such as Lactobacillus acidophilus offers the possibility of a safe, drug-free treatment in the near future," he said.
International experts in gene targeting and mouse genetics present step-by-step protocols for new and experienced researchers in genetics.