acupunctatus contained a bulky germarium and are probably of the meroistic, telotrophic type as reported in other weevils (Grodowitz et al.
(A) Female reproductive system of Scyphophorus acupunctatus formed by 2 ovaries (ov), germarium (g), vitellarium (v), oocyte (o), lateral oviduct (lo), common oviduct (co), spermatheca (s), and genital chamber (gc); (B) Distal part of the germarium (g) with bulky appearance possibly with trophocytes, prefollicular cells, and oogonia, terminal filament (tf), trachea (tr), tracheoles (trl); and (C) Approach to the onset of vitellarium with oocytes in development, germinal vesicle (gv), interfollicular tissue (tif), trachea (tr), and tracheoles (trl).
javanus ovariole (germarium
), indicating cell nuclei that increase in size along the germarium
55 (43-88; n = 12) long, 41 (30-50; n = 12) wide, ovate.
presumably continued to produce eggs that underwent some development in the vitellarium before resorption, as the maximum follicle size did not decrease with time.
Total length 1.35-1.42 mm (1.40 mm), greatest width 221-239 (230) to germarium
Photomicrographs of the female reproductive system of Megamelus scutellaris showing a) distal portion of the ovary showing the distal lateral oviduct (lop), common oviduct (co), bursa copulatrix (b), and spermatheca/spermathecal gland (spt and sptg, respectively), and b) close-up of ovariole morphology (b) showing the anterior lateral oviduct (loa), germarium (g), vitellarium (v), and terminal filament (tf).
a) N1--Note the lack of differentiation in the vitellarium (v) and large size of the germarium (g) in comparison to the vitellarium.
The niche is located in the apex of the oviduct in the bilateral germarium. In general, there are two or three OGSCs in each germarium.
Then, CpCs deliver Hh signaling to AECs (ECs located in the anterior part of the germarium) and promote the transcription of the target genes Decapentaplegic (Dpp) and glass bottom boat (Gbb) (both belong to the Bmp family), in AECs [27, 43].
Gonads overlapping, testis dorsal to germarium. Vas deferens apparently looping left caecum, seminal vesicle a dilatation of vas deferens; prostatic reservoir at level of anterior portion of caeca.
Testis dorsal to germarium, spherical, 42 (31-65, n = 11) in diameter; vas deferens elongate; conspicuous seminal vesicle; prostatic reservoir at level of anterior portion of caeca.