Light microscope observations with the PAS stain showed a strong positive reaction at the site of the egg-stalk of the late active oocyte connected to the follicle wall (germinal epithelium
1-2, 3a-g; 11a), the ovarian germinal epithelium
consists of somatic NPs and germ cells, including amitotic oogonia (AOs); irregularly or conically shaped residual vitellogenic primary oocytes (RVPOs); and a few large residual ova (Os) (Fig.
Spermatogenesis was usually allocated to small regions of the germinal epithelium
, although entire lobes of some gonads were devoted to sperm production (Fig.
Collectively, these observations suggest that an intraovarian oocyte remains arrested at prophase I as long as it is fully encased by a follicular sheath that is connected to the germinal epithelium
Resveratrol and morphine effect on the Sperm parameters, Germinal epithelium
height: Diameter of seminiferous tubules, Testosterone serum level of mice.
Parameters, including tubular diameter of seminiferous tubules, height of germinal epithelium
and germ cell count were noted as per procedure described by Brendtson11 (measurement of seminiferous tubule diameter can be based on only 15 tubules per testis and counts of germ cells in tubule cross sections can be based on only three or four tubules).
ER-immnoreactive cells were found specifically in the germinal epithelium
of the cortex but not in the medulla (Fig.
The germinal epithelium
showed more vacuolation and the cells were disorderly arranged.
It was observed that in the control group, Galectin1 expression was weak staining in germinal epithelium
and moderate staining in basement membrane and tunica albuginea layer (Fig.
Morphometric study was performed on tissue from both the control and experimental animals to detect the diameter of seminiferous tubule, (DST) height of germinal epithelium
(HE) and thickness of interstitial tissue (TI).
For the histometric analysis of the testicular tissue an optic microscope was connected to a digital microscopy camera and 10 microscope fields were captured per tissue sample, image analysis software (Motic Images Plus[R], version 2.0) was used to measure the germinal epithelium
, the intertubular interstitium and the diameter of the seminiferous tubules found in each microscopic field.
Student paired t-tests were used to compare between paired groups, whereas the one way analysis of variance (ANOVA) was used to compare the differences in weight, volume, circumference and length of testes, seminiferous tubule diameter, height of germinal epithelium
across the groups.