Meiotic maturation rate of oocytes injected with Xpress-Egr3-DsRed2 or Xpress-[DELTA]Egr3-DsRed2 cRNA (1) Group Total Oocytes maturation rate (%) oocytes GVBD MII oocytes (%) oocytes (%) Xpress-Egr3-DsRed2 85 84 (98.82) 51 (60.00) Xpress-[DELTA]Egr3-DsRed2 78 74 (94.87) 52 (66.66) Water injection 40 40 (100) 29 (72.50) No injection 68 65 (95.59) 54 (79.41) GVBD, germinal vesicle
breakdown; MII, metaphase II; IBMX, 3-isobutyl-1-methylxanthine.
Follicles containing oocytes with germinal vesicles
are surrounded by a few layers of basketlike capillary wreaths that lie adjacent to the follicular basement membrane.
Percentage of nucleus which reached metaphase II oocytes in TCM199 medium +FCS10% + 10% GFF was caused germinal vesicle
fusion can accelerate and enable the process of reduction division nucleus.
Isolation of GV Oocytes: Germinal vesicle
oocytes were obtained from 8-10 week old female mice (n=40) who were primed with an intraperitoneal injection of 7.5 IU pregnant mare's serum gonadotropin (PMSG; Folligon[R], Intervet, Castle Hill, Australia) 48 hr prior to oocyte retrieval.
AMP-activated kinase; ASW, artificial seawater; CaFSW, calcium-free seawater; ER, endoplasmic reticulum; GMP, guanosine monophosphate; GV, germinal vesicle
: GVBD, germinal vesicle
breakdown; [IP.sub.3], inositol 1.4,5-trisphosphate: MAPK, mitogen-activated protein kinase; MPF, maturation-promoting factor; NO, nitric oxide; NOS, nitric oxide synthase: PDE, phosphodiesterase; PKA, protein kinase A; PKC, protein kinase C; PLC, phospholipase C: SFK, Src family kinase; SW.
Photomicrograph of the female reproductive system of Megamelus scutellaris showing ovaries (ov), common oviduct (c), anterior and posterior portions of the lateral oviduct (loa and lop, respectively), and overall structure of a follicle including the germinal vesicle
(gv) and oocyte with yolk (oy).
We investigated several such factors including germinal vesicle
(GV) chromatin configuration, CG migration, mitochondrial distribution, AcH3K9 levels, and apoptotic nuclear features of BCB+, BCB-, and control oocytes.
The females which are capable of spawning are those which appear during the reproductive cycle with vitelogenic oocytes or with histological indicators of imminent spawning (late migration of germinal vesicle
, germinal vesicle
breakdown and hydration) or recent spawning (POFs of recent collapse).
Methods: The oocytes at Germinal Vesicle
(GV) and Metaphase ll (MI1) stages were recovered from 6-8 week.
Three phases of spawning-capable (SC) ovaries were assigned in the histological index as 3.1, 3.2, and 3.3 to differentiate the process of early, middle, and late phases of OM: early germinal vesicle
migration (GVM) and germinal vesicle
breakdown (GVBD) (Table 2).
In vitro maturation and fertilization of goat oocytes frozen at the germinal vesicle
During oogenesis, animal oocytes usually arrest at prophase I of meiosis and develop an enlarged nucleus (germinal vesicle