Cooking Phaseolus bean varieties changes their albumin, globulin (including phaseolin), prolamin, and glutelin
distribution, the solubility decreases for all four fractions, and the extent to which solubility was affected varied with the type of protein.
As shown previously, the most abundant fraction in nut was glutelin
. A total of 21 peptides with different biological activities were found: (a) in the glutelin
fraction, peptides with antihypertensive, antioxidant, and anticarcinogenic activities were detected.
This factor is based on the nitrogen content (16.8%) of glutelin
, the main protein of rice (Juliano et al., 1985).
The IVPD of the glutelin
fraction increased during the last six hours of fermentation.
fraction remaining in the meal was then extracted with 0.05 M NaOH for 30 min.
The non-zein protein consists of globulins (3%) glutelins
(34%) and albumins (3%).
After removing albumin, globulin, and prolamin, the residue was extracted with 500 mL of 0.01 M NaOH according to the procedures described for the extraction of albumin to obtain the glutelin
This common extraction methods used in this study is feasible as rice bran protein consisted of albulmin: globulin: glutelin
at the ratio of 36:5:22 with mainly albumin and globulin (Juliano, 1985).
This research has indicated, for example, that many ribosomal proteins and histone genes were found in growth-phase callus while genes encoding globulin and seed storage proteins such as glutelin
and prolamine were identified in ripening panicles.
The chief protein fractions of CGM are zein and glutelin
, which constitute approximately 65% (w/w) and 30% (w/w), respectively.
Traditionally, the extraction of storage proteins from seeds has been carried out following Osborne  who classifies proteins according to their solubility in water (albumin), salt solutions (globulin), alkali solutions (glutelin
), and alcohol solutions (prolamin).
Quantitative nature of the Prolamin-box, ACGT and AACA motifs in a rice glutelin
gene promoter: Minimal cis-element requirements for endosperm-specific gene expression.