XBP1, X-box binding protein 1; usXBP1, un-spliced XBP1; sXBP1, spliced XBP1; RT-PCR, Reverse transcription polymerase chain reaction; qPCR, quantitative real-time polymerase chain reaction; ANOVA, analysis of variance; HSD, honestly significant difference; GAPDH, glyceraldehyde
ELISA: enzyme-linked immunosorbent assay; GAPDH: glyceraldehyde
3-phosphate dehydrogenase; NF-[kappa]B: nuclear factor kappa B; TNF-[alpha]: tumor necrosis factor-alpha.
Table 3 shows the results of the docking study between compounds 1-12 with the enzyme glyceraldehyde
(ANOVA followed by Bonferroni's test.) BZYQD: Bu-Zhong-Yi-Qi decoction; LC3: microtubule-associated protein light chain-3; GAPDH: glyceraldehyde
3-phosphate dehydrogenase; 3-MA: 3-methyladenine.
MicroRNA genes were measured relative to U6 and other genes relative to the housekeeping gene for glyceraldehyde
ADAMTS12 Forward AGTGGGCAACTGGAGTGAGT 67 bp product Reverse ACATGTGACACTGCGAATCC GAPDH Forward CCTGCACCACCAACTGCTTA 108 bp product Reverse TCTTCTGGGTGGCAGTGATG ADAMTS: A disintegrin-like and metalloproteinase with thrombospondin motifs; GAPDH: Glyceraldehyde
GAPDH detoxifies As using arsenate instead of phosphate converting glyceraldehyde
3-phosphate into 1-arseno-3-phospho-glycerate.
Crystal structures of P falciparum glycolytic enzymes aldolase (PfALDO) , triosephosphate isomerase (PfTPI) , glyceraldehyde
3-phosphate dehydrogenase (PfGAPDH) , lactate dehydrogenase (PfLDH) , glucose 6-phosphate isomerase (PfGPI) (Gileadi et al.
The synthesis of title compound initiates from a wellknown carbohydrate precursor (R)-2,3-isopropylidene glyceraldehyde
5, which can be easily prepared from commercially available D-mannitol [23-26].
Con: control; BOO: bladder outlet obstruction; GAPDH: glyceraldehyde
The genes of interest were glyceraldehyde
3-phosphate dehydrogenase (GAPDH, control), osteocalcin (BGLAP), transcription factor RunX2 (RunX), alkaline phosphatase (AP), and SMAD family member 4 (SMAD4) (Table 1).
The glycolytic enzyme glyceraldehyde
3 phosphate dehydrogenase mediates nuclear cell death.