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The science involved with maintaining a microbiologically controlled environment, and with the knowledge necessary to obtain and use biological specimens in this environment. The roots of the word are gnotos, meaning well known, and biota, the combined flora and fauna of a region.

All exposed surfaces of an animal are teeming with microbes. For example, the contents of the large intestine may contain 3 trillion microbes per ounce (100 billion per gram), belonging to several hundred species. Even if the animal itself is the primary interest of a researcher, there is no direct way to determine how many of an animal's normal characteristics are truly its own, and how many involve interaction with or reaction against resident microbiota. The only way to determine this is comparison with animals that have no microbiota. If differences are found, then the role of individual microbial species can be studied by inoculating pure cultures of these species into the animals without a microbiota.

Thus, gnotobiotics evolved initially to answer questions about what difference the resident microbiota makes, and which members of the microbiota make the difference. Answers become more and more essential in going beyond the effects of pathogenic microbes to the harmful or helpful long-term effects of environmental chemicals, compounds produced in the host's own metabolism, and therapeutic drugs being tested for efficacy, toxicity, or carcinogenicity. The activity of the microbiota is proportionately much greater in laboratory animals than it is in humans, and could have a decisive effect on such chemicals, especially since the chemicals are often received in small doses. For example, intestinal microbes turn a minor component of the cycad bean, a South Pacific foodstuff, into a carcinogen. One of the best drugs against parasitic schistosomes in humans is turned into a carcinogen by a single species of intestinal streptococcus. Gnotobiotic studies are designed to detect such possibilities.

Gnotobiote is the term applied to an animal (or plant) with a defined microbiota. The most simple of gnotobiotes is the animal with no microbiota. Invertebrate animals of this type are most frequently called axenic. Vertebrate animals may also be called axenic, but are more frequently called germfree. Gnotobiology is a term sometimes used to designate studies involving gnotobiotes, although it tends to suggest that there is a unified body of knowledge which results from studying gnotobiotes. In fact, the gnotobiote is a more precisely defined laboratory animal which helps elucidate biological phenomena in immunology, nutrition, physiology, oncology, gastroenterology, microbial ecology, gerontology, pathogenic microbiology, parasitology, and so on.

McGraw-Hill Concise Encyclopedia of Bioscience. © 2002 by The McGraw-Hill Companies, Inc.
The following article is from The Great Soviet Encyclopedia (1979). It might be outdated or ideologically biased.



gnotobiology, a branch of experimental biology that includes the production and raising of laboratory animals whose bodies are free either of all microorganisms (including bacteria, viruses, protozoans) and helminths and arthropods (so-called sterile animals) or merely of certain pathogenic species of them (so-called gnotobiotic animals). Gnotobiotics arose with the requirements of morphology, physiology, genetics, microbiology, medicine, veterinary medicine, and other sciences.

The Great Soviet Encyclopedia, 3rd Edition (1970-1979). © 2010 The Gale Group, Inc. All rights reserved.


The science involved with maintaining a microbiologically controlled environment, and with the knowledge necessary to obtain and use biological specimens in this environment.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.
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in the Gut of Gnotobiotic Mice." Antimicrob Agents Chemother, 50(3): 1054-1062.
In general, the inadequately functional immune system of gnotobiotic mice or neonates suggests that its maturation is compelled by the resident microbiome.
Another strategy that circumvents both gnotobiotic and antibiotic models is surgery to create isolated intestinal segments, such as Thiry-Vella fistulas, to determine the effect of autonomous microbial changes on intestinal function without experimental modification of the lumen [193].
Lactobacillus rhamnosus GG on rotavirus-induced injury of ileal epithelium in gnotobiotic pigs.
Effect of probiotic bacteria on induction and maintenance of oral tolerance to AY-lactoglobulin in gnotobiotic mice.
Periodontal bone level and gingival proteinase activity in gnotobiotic rats immunized with Bacteroides gingiva/are.
The obesity-inducing capacity of this human-derived endotoxin producer in gnotobiotic mice suggests that it may causatively contribute to the development of obesity in its human host." (2)
Currently, colonization by more than several tens of intestinal microbes isolated from indigenous microbiota is thought to be necessary to elevate the colonization resistance and IgA response in gnotobiotic mice (26,27) to the levels of CV ones because normalization of the cecal size and other physiological and histological characteristics of GF animals by association with one or a few kinds of bacterial species has not been successful.
Scoring microscopic lesions in the bursa of fabricius thymus spleen and kidney in gnotobiotic and battery reared White Leghorns experimentally infected with infectious bursal disease virus.
Immunostimulatory nature of beta-glucans and baker's yeast in gnotobiotic Artemia challenge tests.
The gnotobiotic animal as a tool in the study of host microbial relations helps.