In Pakistan initial field evaluation of EPN is likely to be done with in vivo produced nematodes in hosts such as greater wax moth
Impact of soil texture on the infectivity of different species of Entomopathogenic nematodes against Greater Wax moth
(Galleria mellonella L).
The greater wax moth
or honeycomb moth, Galleria mellonella L.
The Greater Wax Moth
, Galleria mellonella (L.), is a worldwide serious honey bee pest, especially in warm climate regions of the world (Calvert, 1982; Hachiro and Knox, 2000).
Steinernematid, Heterorhabditid, Emergence, Greater wax moth
, Infective juveniles.
A technical hurdle that's kept the insect-cadaver approach from gaining widespread commercial acceptance is the tendency of some commonly used host insects--notably the soft-bodied greater wax moth
larvae--to rupture or stick together during storage, transport, and application.
When the parasitic nematode Heterorhabditis bacteriophora infects caterpillars of the greater wax moth
, the normally pale caterpillars turn persistently pink-red and temporarily luminescent.
Galleria mellonella (L.) (Lepidoptera: Pyralidae), known as the greater wax moth
or honeycomb moth, is a serious pest for the apiculture industry.
During early research, they used cadavers of Tenebrio molitor, commonly known as the yellow mealworm, and Galleria mellonella, or greater wax moth
. The nematode-infected hosts are first dipped in a liquid mixture and then rolled in a powder.
In Pakistan, techniques for mass production of entomopathogenic nematodes (EPN) are not yet available and the development and use of EPN mainly depends on the use of host insects such as greater wax moth
(Galleria mellonella) for in vivo production.
The larvae, pupae and adults of the host, greater wax moth
hebetor (Say.) (Hymenoptera: Braconidae) was reared in the laboratory on the late stage (5th instars) larvae of greater wax moth