Hematoxylin

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hematoxylin

[‚hē·mə′täk·sə·lən]
(organic chemistry)
C16H14O6 A colorless, crystalline compound occurring in hematoxylon; upon oxidation, it is converted to hematein which forms deeply colored lakes with various metals; used as a stain in microscopy.

Hematoxylin

 

a dye used in microscopy for staining plant and animal tissues. Hematoxylin is extracted by ether from the colored wood of the logwood tree, which is native to Central America and the Antilles Islands. In the course of its preparation for use in microscopy, the substance is “matured,” or oxidized to hematein, which stains cell nuclei, chromosomes, and cell membranes a blue or blue-black color.

REFERENCE

Romeis, B. Mikroskopicheskaia tekhnika. Moscow, 1953. (Translated from German.)
References in periodicals archive ?
Haematoxylin and Eosin stain, B & E- G (scale bar=100 [micro]m).
(a) Haematoxylin and eosin staining shows a low-power view with a nest of invasive adenocarcinoma into the pericolic adipose tissue.
Caption: Figure 5: Paraffin sections stained by haematoxylin and eosin (H&E) x400 for histomorphometry examination of brain (cerebrum) tissues of rats as follows.
On the rapid conversion of Haematoxylin into haematein in staining reactions.
Treatment with aloin (0.75 pM) prevented multinuclear formation (haematoxylin and eosin staining), reduced intracellular TRAP content (TRAP Staining) and increased F4/80 content (F4/80 immunohistochemistry) in RANKL (20 ng/ml) treated RAW cells.
[3] The granulomatous appearance on haematoxylin eosin staining is that of connective tissue with dense mononuclear infiltrate (Fig.
Multiple tissue sections were embedded from different parts and were stained with haematoxylin and eosin.
(Haematoxylin staining was used as far back as 1758 on plant tissues; and after more than a century of medical use, the Haematoxylin/Eosin, or "H&E" staining pair, continues as a first choice mainstay to this day, recognizable to even many non-specialists.)
Sections of about 6 m thickness were cut, dewaxed in xylene, hydrated in series of descending ethanol concentration and routinely stained with haematoxylin and eosin (Wilson and Gamble, 2005).
The use of light microscopy alone does not identify all substances on examination of a Haematoxylin and Eosin (H&E) stained section of tissue.
The examination of formalin-fixed tissues with haematoxylin and eosin staining has been the diagnostic method of choice, showing cryptosporidians as 2.0-7.5[micro]m basophilic bodies within the brush border of the epithelial cells (SRETER & VARGA, 2000).