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A 20 [micro]L aliquot of the stained cell suspension was removed, and the released nuclei were counted with a haemocytometer as a measure of the culture cell density.
cerevisiae cell number was counted with a haemocytometer. The final ethanol content was measured by GC analysis.
Cells were counted using a haemocytometer and plated in 75 [cm.sup.2] flasks (7.5 x [10.sup.5] cells) for further culture, or onto 6-well and 24-well plates (1 x [10.sup.5] and 2 x [10.sup.4] cells per well, resp.) for experiments.
To establish the inductions, a confluent culture of undifferentiated 46C cells was dissociated with 0.25% trypsin, and the cell suspension was counted using a haemocytometer. Approximately 5 x [10.sup.6] cells  were seeded in a 100 mm bacteriological grade Petri dish (non-tissue culture) in 10 mL of medium (standard medium as mentioned above) without LIF.
Treatment was done for 24 hours, under 17 [micro]mol photon m-2 s-1, 25[degrees]C and the cell number at 0h, 12h, 24h of treatment was taken using haemocytometer and trypan blue stain for death cell.
Erythrocytes (RBCs) count m/mm3 calculation was made by Haemocytometer (Kolmer et al., 1986) method.
Concentration of bacterial cells is measured by Haemocytometer and optical density could be found by spectrophotometer analysis before adding bacteria.
(2) Yeast cell count was carried out using a haemocytometer, and all statistical analysis was carried out using XLSTAT 2014.
The apparatus used in this research work included glass tools which are generally used in the laboratory, jars made of cover glass, aerator, salinometer, centrifuge, haemocytometer, Japan Nikon microscopes SE model type 102, Olympus microscope SZX16, desiccators, pumps vacuum, Buchner funnel, water bath, water bath, Butchi rotary evaporator, blower, Oswald viscometer, burette 50 mL Pyrex, analytical balance, and ultrasonic equipment S 40 H Elmasonic.
Percentage of colonies reduction from bacteriostatic effect was determined based on the bacterial colonies calculation using haemocytometer by the following equation:
The sample was centrifuged and the cell pellet were resuspended in 2 mL of the same medium with 1% FCS, for total (after dilution in Turk's stain) and differential (after staining for eosinophil peroxidase; [27, 28]) counts on haemocytometer and cytocentrifuge slides, respectively.
The SRB growth curve was attained by counting the bacteria cells under a microscope at 400x magnification and using a haemocytometer method for ATCC 7757 and Sg.
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