After 24 h marinating the 200 KDa polypeptides (heavy meromyosin
) were identified in all samples (Figure 3).
Expression and purification of full-length of Physarum myosin, short-tailed heavy meromyosin, and Physarum myosin with mutant Ca-binding light chain.
(2007) Calcium inhibition of Physarum myosin as examined by the recombinant heavy meromyosin. In Regulatory Mechanisms of Striated Muscle Contraction (eds.
Squid mantle muscle contains endogenous metalloproteinases that selectively degrade myosin molecules into heavy meromyosin (HMM) and light meromyosin (LMM) (Yoshioka et al., 2005).
Yoshioka T, Kinoshita Y, Kato S, Cho Y, Konno K (2005) Preparation of heavy meromyosin from the autolyzed squid mantle homogenate.
N-Ethylmaleimide modified heavy meromyosin
: a probe for actomyosin interactions.
Stepwise motion of an actin filament over a small number of heavy meromyosin
molecules is revealed in an in vitro motility assay.
Estimation from slow sliding movement of actin over low densities of heavy meromyosin