hemacytometer


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hemacytometer

[‚hē·mə·sī′täm·əd·ər]
(pathology)
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0),1 mmol/L EDTA] and enumerated by use of either a Beckman Coulter Z2 Particle Count and Size Analyzer or a Neubauer Bright-Line Hemacytometer.
Conidia were counted with a hemacytometer, and the inoculum was standardized to 1.
Moreover such methods often produce variable results since they rely on inherently subjective visual discrimination of dye-stained cells using a hemacytometer and microscope.
Traditionally, body fluid counts have been performed by manually counting RBCs and nucleated cells under a microscope using a hemacytometer.
The resultant cell pellet was resuspended in 300 [micro]L of PBS and 20 [micro]L was removed for hemacytometer counts.
The red blood cell (RBC), white blood cell (WBC) and thrombocytes total counts were performed manually by using NattHerrick diluent and a Neubauer hemacytometer.
Sperm counts, abnormal cells (malformations of the main body or absence of the spike), and dead cells (blue coloration) were estimated with cell counts, using a hemacytometer under a light microscope.
The concentrations of viral occlusion bodies (OBs) were quantified with a hemacytometer and stored in aliquots of 500 [micro]l of distilled water at 0[degrees]C until required.
BALF was centrifuged (200 x g for 10 min at 4[degrees]C) to remove the supernatant, the cells were resuspended in 1 ml of HBSS, and total leukocytes in BALF counted manually with a hemacytometer.
At the end of the culture period, the number of cells per well was estimated after trypsinization with a hemacytometer under a phase contrast microscope.
When using the hemacytometer to perform cell counts, the dilution and number of squares counted should be adjusted for the number of nucleated cells present, which may be estimated from the specimen's clarity.