Daily algae culture density was determined before feeding, with cell counts obtained using a
hematocytometer.
In the past few decades, automated hematology analyzers have replaced manual
hematocytometer blood-cell counts with increased reliability, precision, and accuracy.
The length (cm) and height (cm) of angelfish larvae samples were first measured using a
hematocytometer under a stereoscopic microscope.
Cell viability was determined microscopically by trypan blue exclusion, and the number of cells was counted by a
hematocytometer.
Following a cell count (standard procedure under a microscope with a
hematocytometer), we were able to determine the cell concentration of our solution, which was then adjusted with medium to obtain 4 mL of solution with a concentration of 1 700 000 cells/mL.
Confluent LNCaP cells were harvested, washed, and counted with a
hematocytometer; we then diluted [10.sup.6] cells resuspended in 1 mL of RPMI 1640 in 10-fold serial dilutions.
The cell concentration of each strain was quantified by direct cell count every 48 h during 10 days, using a
hematocytometer (0.1 mm depth), and a compound microscope.
