1] transgenic plants were expected to be either homozygous or hemizygous
for the inserted transgene(s) (Fig.
Finally, deletions of one or more exons, estimated to occur at a frequency of 1%-3% in disease-causing chromosomes (35), will not be detected and could make the scanning of hemizygous
variants within the deleted area more difficult.
CryIA-positive plants derived from MON 81 or MON 249, that were either homozygous or hemizygous
for a cryIA insert, were analyzed in a completely randomized design.
To assess the suitability of this method for the follow-up of Gaucher and Fabry patients during therapy, we investigated samples from 4 Gaucher patients, 2 on ERT and 2 on SDT, and 2 hemizygous
Fabry patients on ERT.
Finally, one primer (4%) detected three alleles in Obino l'Ewai, which may indicate a combination of both heterozygous and hemizygous
forms of homoeologous loci in the A and B genomes, respectively.
The method would therefore be amenable to screening an X-linked dominant disorder with heterozygous females and hemizygous
This indicated the A fragment was hemizygous
and the monosome in these plants was a P-genome chromosome.
Classically, it affects hemizygous
males with no residual [alpha]-galactosidase A activity, displaying all the characteristic signs of the disease.
HIF [sup]flox/- mice were bred with muscle creatine kinase ( MCK )- Cre [sup]+ hemizygous
mice to create HIF [sup]flox/- Cre [sup]+ mice.
Molecular analysis revealed two novel hemizygous
mutations in the patients: a deletion mutation (c.
minipigs produced by random gene insertion and handmade cloning express the Alzheimer's diseasecausing dominant mutation APPsw.
Since males are hemizygous
for chromosome X, only maternal haplotype analysis and fetal inheritance of the maternal X-linked mutations were performed (Fig.