A PRP sample and a whole blood sample were sent to the laboratory for platelet count in Neubauer's hemocytometer
and confirmation of PRP concentration (three to five times higher than in whole blood) prior to use of the product inactivated in the cell culture.
The BD system isolates cells in the hemocytometer
adapter's microwells, each of which is bar coded.
The spermatozoa was counted by hemocytometer
using the improved Neubauer (Deep 1/ 10 mm, LABART, Germany) chamber as described by Pant & Srivastava (2003).
Sperm concentration was determined using a hemocytometer
as described by Kaka et al.
We also counted the free-swimming algae (in some cases using a hemocytometer
) and estimated the concentration of algae in HCS.
The concentration of conidia was estimated using a Neubauer hemocytometer
and adjusted to 1 x [10.sup.8] conidia per mL.
Cell density was determined by using hemocytometer
and was kept 1x106 cells/mL.
Cell suspension then transferred to sterile tube in order to pull 25 [micro]L from it into eppendorf tube that contains 25 [micro]L trypan blue dye, to count the number of cells that found in the suspension by using hemocytometer
according to the following formula: (Number of cells=average number of cells from each of the sets of 16 corner squares of hemocytometer
x 2 (dilution factor) x 10000 x volume of cell suspension).
The blood samples were subjected to analysis of Hemoglobin (gm%) by Sahlis hemometer method, Packed cell Volume (PCV%) by microhematocrit method, Total red blood cell count (millions/dl of blood) and total white blood cell count (thousands/dL of blood) by Neubars hemocytometer
and blood smears prepared on grease free glass slides were stained with Leishmans stain for differential leucocyte count.
The contents were shaken well and a volume of 0.25ml of liquid containing the pollen was flown underneath the cover slip of the hemocytometer
. Number of pollen grains present in the five small squares was recorded following procedure explained by Fuentes .
Ameba density was determined with a hemocytometer
according to Lopez-Revilla and Rodriguez-Baez  before being reseeded.
Sampled cells were counted with a hemocytometer
using trypan blue exclusion to determine cell viability.