Try to avoid bubbles within the
hemocytometer and cover-slip.
Subsequently, the wells were rinsed twice with DPBS and then the percentage of the AP-positive cells was counted by a
hemocytometer.
Cells were counted before injection using the bright line
hemocytometer and dilutions were made by physiological saline, and the desired number of cells was injected in a volume of 0.
Sperm count was done under the microscope using a new improved Neubauer
Hemocytometer and the sperm count was calculated.
At confluency, cells were trypsinized (Hyclone EDTA-Trypsin), removed from the flask and counted by the use of a Neubauer
hemocytometer.
Cells were counted using a
hemocytometer and reseeded in flasks with pre-warmed growth medium at approximately 100,000 cells per 75[cm.
To test this model, we utilized knockout mice and enumerated hematopoietic bone marrow cells from femur washes by manual cell counting on a
hemocytometer visualized by light microscopy.
After 48 h of growth in media, cultures were counted using a
hemocytometer (Hausser Scientific, Horsham, PA) and diluted to 50,000 cells/ml in fresh culture medium and transferred to 50 ml culture flasks (10 ml/flask).
After counting with a
hemocytometer, approximately 4,000 cells were plated on glucose medium agar plates containing resveratrol (positive control, 10 [micro]M) or parishin (0, 3, 10, and 30 [micro]M).
Dilute an aliquot of the spore suspension as needed for cell counting with
hemocytometer.
Briefly, to 100 [micro]l of hemolymph, 100 [micro]l of Anti Aggregant Solution for Hemocytes (AASH) was added (Auffret & Oubella 1995), cells were counted in both sides of a Neubauer
hemocytometer using a phase microscope (40x).
6] trypanosomes/mL, as determined by the
hemocytometer method [25], the other animals (named from 1 to 5) were inoculated intravenously with 2 mL of sheep donor fresh blood (approximately 2 x [10.
