A PRP sample and a whole blood sample were sent to the laboratory for platelet count in Neubauer's
hemocytometer and confirmation of PRP concentration (three to five times higher than in whole blood) prior to use of the product inactivated in the cell culture.
The BD system isolates cells in the
hemocytometer adapter's microwells, each of which is bar coded.
The spermatozoa was counted by
hemocytometer using the improved Neubauer (Deep 1/ 10 mm, LABART, Germany) chamber as described by Pant & Srivastava (2003).
Sperm concentration was determined using a
hemocytometer as described by Kaka et al.
We also counted the free-swimming algae (in some cases using a
hemocytometer) and estimated the concentration of algae in HCS.
The concentration of conidia was estimated using a Neubauer
hemocytometer and adjusted to 1 x [10.sup.8] conidia per mL.
Cell density was determined by using
hemocytometer and was kept 1x106 cells/mL.
Cell suspension then transferred to sterile tube in order to pull 25 [micro]L from it into eppendorf tube that contains 25 [micro]L trypan blue dye, to count the number of cells that found in the suspension by using
hemocytometer according to the following formula: (Number of cells=average number of cells from each of the sets of 16 corner squares of
hemocytometer x 2 (dilution factor) x 10000 x volume of cell suspension).
The blood samples were subjected to analysis of Hemoglobin (gm%) by Sahlis hemometer method, Packed cell Volume (PCV%) by microhematocrit method, Total red blood cell count (millions/dl of blood) and total white blood cell count (thousands/dL of blood) by Neubars
hemocytometer and blood smears prepared on grease free glass slides were stained with Leishmans stain for differential leucocyte count.
The contents were shaken well and a volume of 0.25ml of liquid containing the pollen was flown underneath the cover slip of the
hemocytometer. Number of pollen grains present in the five small squares was recorded following procedure explained by Fuentes [48].
Ameba density was determined with a
hemocytometer according to Lopez-Revilla and Rodriguez-Baez [22] before being reseeded.
Sampled cells were counted with a
hemocytometer using trypan blue exclusion to determine cell viability.
